Identification by monoclonal antibody of a major (28 kDa) surface membrane antigen of Schistosoma mansoni |
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| Authors: | D A Harn M Mitsuyama E D Huguenel L Oligino J R David |
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| Affiliation: | Department of Rheumatology and Immunology, Division of Tropical Medicine, Brigham and Women''s Hospital, and Department of Medicine, Harvard Medical School, Boston, MA 02115, U.S.A. |
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| Abstract: | Monoclonal antibody M.1 was generated from mice immunized with membrane enriched extracts of mechanically transformed schistosomula. M.1 bound to the surface membranes of cercariae and young (0-24 h post-transformation) schistosomula but did not bind to older schistosomula or cultured worms. M.1 immunoprecipitated an antigen of approximate molecular weight 27-28 kDa from schistosomula. Experiments using metabolic labeling showed that the antigen was actively synthesized by developing schistosomula. Further M.1 immunoprecipitated a similar 27-28 kDa antigen from membrane-enriched extracts of miracidia, lung and adult worms as well as from schistosomula. |
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| Keywords: | Monoclonal antibody Surface membrane antigen ELISA enzyme-linked immunosorbent assay PBS phosphate buffered saline NP-40 Nonidet P 40 SDS sodium dodecyl sulfate 2-D two-dimensional |
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