Correlative analysis of neoplasm patients with phlegm-stasis(痰瘀) or abnormal savda(异常黑胆质) syndrome,based on metabonomics

OBJECTIVE:To investigate plasma samples from neoplasm patients with phlegm-stasis or abnormal Savda syndrome,with NMR spectroscopy,and to analyze their metabolic varieties,characteristics and reciprocity.METHODS:1 H-NMR spectra were analyzed using the orthogonal projection to latent structure with discriminative analysis(OPLS-DA) method with unit variance scaling.The discriminative significance of metabolites was determined by the Pearson’s product-moment correlation coefficient.RESULTS:Compared to the control group,neoplasm patients with phlegm-stasis or abnormal Savda syndrome had low concentrations of leucine,isoleucine,valine,alanine,tyrosine,histidine,citrul-line,glycoprotein,glutamine,myo-inositol,scyllo-inositol,creatine,α-glucose,β-glucose and lactate(P<0.05),and high concentrations of very low density lipoprotein,low density lipoprotein,unsaturated lipid,formate,acetone,acetate,acetoacetate,pyruvate,β-hydroxybutyrate,carnitine and malonic acid(P<0.05),with no significant differences between the phlegm-stasis and abnormal Savda syndrome patients.CONCLUSIONS:Neoplasm patients with different syndromes have very similar metabolic changes.A series of abnormalities such as immune dysfunction and oxidative-antioxidative imbalance,occur in neoplasm patients with abnormal Savda or phlegm-stasis syndrome.