胆道梗阻及胆肠内引流对肿瘤坏死因子α影响的实验研究

以大鼠胆总管结扎及胆肠内引流模型，观察胆道梗阻及早期胆肠内引流后血清肿瘤坏死因子α（ＴＮＦ α）、肝功能及肝脏形态学的改变。结果显示：胆总管结扎后血清ＴＮＦ α、ＧＰＴ值及肝组织羟脯氨酸含量升高，血清白蛋白水平降低；早期胆肠内引流后血清ＴＮＦ α、ＧＰＴ值及肝组织羟脯氨酸含量下降，血清白蛋白水平升高；血清ＴＮＦ α含量与血清ＧＰＴ、白蛋白及肝组织羟脯氨酸含量呈相关关系。结果表明ＴＮＦ α与胆道梗阻所致的肝功能损害和肝纤维化有关；早期胆肠内引流能降低ＴＮＦ α水平，促进肝功能恢复，减轻肝纤维化程度，防止肝硬变形成。

Changes in Production of Tumor Necrosis Factor α Associated with Biliary Obstruction and Biliary Drainage in Rats

his study was undertaken to evaluate the alteration in serum Tumor necrosis factor α (TNF α) level, liver function and liver morphology in rats during the biliary obstruction and biliary drainage. The results have demonstrated that: after the biliary obstruction, there was an increase of serum GPT level, serum TNF α level and liver hydroxyproline content and a decrease of serum albumin level; abnormalities in a liver function test, serum TNF α level and hepatic collagen content resulting from biliary obstruction were significantly improved by bilioduodenal anastomosis after a two week biliary obstruction; serum TNF α concentration strongly correlated with serum GPT level, serum albumin level and liver hydroxyproline content, respectively. These findings show that: TNF α may be involved in the pathogenesis of liver function impairment and liver fibrosis; bilioduodenal anastomosis at an early stage of biliary obstruction can improve hepatic function, reduce the degree of liver fibrosis, and prevent liver cirrhosis.