Proteins with insulinlike activity isolated from oyster (Ostrea edulis L.) hepatopancreas |
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| Authors: | N R De Martínez M C García M Salas J L Candela |
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| Affiliation: | Departamento de Biología Molecular, Instituto G. Marañón, C.S.I.C. Velázquez 144, Madrid 6, Spain |
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| Abstract: | Three radioactive protein peaks, I, II, and III, with insulinlike activity, have been obtained from oyster hepatopancreas by chromatography in Biogel-P30 columns. The molecular weight values for the peaks were 14,000, 11,000, and 7000, respectively. Trypsin treatment transformed peak II into peak I. Peak I was transformed into peak III by incubation at pH 9. The insulinlike activity of the three peaks was decreased by treatment with cysteine. The presented results suggest that peaks I, II, and III contain insulin dimer, proinsulin, and insulin, respectively. |
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