刺五加叶柄的体细胞胚胎发生研究

目的 以来源广泛、原植物药用成分量可测定的刺五加叶柄为外植体，研究刺五加体细胞胚胎发生情况，为实现药用成分高产刺五加的快速繁殖奠定基础。方法 以 3 年生植株萌发 15 d 以内的刺五加复叶叶柄为外植体，考察 2，4- D 和 BA 对刺五加体细胞胚胎发生的影响。结果 28 d 培养后，2, 4-D 1.5 mg/L+BA 1.0 mg/L 处理的叶柄外植体中 71.4% 直接产生或经由愈伤组织间接产生了 8.5 个体胚。两种产生方式均可在诱导培养基中进行，但间接发生比例较少。转入相同或降低 2，4-D 质量浓度的培养基后，体胚渐次发育成熟。同时新的体胚也在逐渐产生，经由间接途径产生的体胚所占比例也随之上升。结论 刺五加可以通过萌发 15 d 以内的复叶叶柄外植体实现体细胞胚胎发生，体胚诱导率取决于 2，4-D 和 BA 的质量浓度。

Somatic embryogenesis from petiole in Eleutherococcus senticosus

Objective The petiole of Eleutherococcus senticosus was used as somatic embryo, which is widely original plants with the pharmacological active components whose contents could be determined, the somatic embryo in E. senticosus was studied, the aim of this study is to provide the proof of E. senticosus species which have the higher yield of pharmacological active components. Methods Using the petiole of E. senticosus of three years old plants germinated somatic embryos within 15 d to observe the somatic embryogenesis of E. senticosus with the 2,4-D+BA medium. Results After cultured for 28 d with 2, 4-D 1.5 mg/L+BA 1.0 mg/L, 71.4% of the petiole somatic embryos were directly produced or 8.5 embryos in total were produced via callus. Both of the two methods could be used in the elicitation medium, but the percentage of indirect production was smaller. After transforming into the same or the lower concentration of 2,4-D medium, the somatic embryos gradually matured. At the same time, those of the new somatic embryos were also produced, the percentage of the somatic embryos which were produced by indirect way was increased with it. Conclusion Using the petiole of E. senticosus germination within 15 d could make somatic embryogenesis. It confirms that the somatic embryogenesis and the bodybmeryos inductivity depend on the 2, 4-D and BA concentration.