| HIF在兔角膜碱烧伤后CNV生成中的作用研究 |
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| 引用本文： | 胡琦,王建文,崔美芝,王珂萌,周文艳,徐洋涛,罗杰,俞佳伟.HIF在兔角膜碱烧伤后CNV生成中的作用研究[J].眼科新进展,2011(7):612-614. |
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| 作者姓名： | 胡琦 王建文 崔美芝 王珂萌 周文艳 徐洋涛 罗杰 俞佳伟 |
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| 作者单位： | 哈尔滨医科大学第一附属医院眼科视光学中心; |
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| 基金项目： | 黑龙江省教育厅科学技术研究项目(编号:11521159)~~ |
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| 摘 要： | 目的研究缺氧诱导因子(hypoxia inducible factor,HIF)以及诱导性一氧化氮合成酶(inducible nitric oxide synthase,iNOS)和血管内皮生长因子(vascular endothelial growth factor,VEGF)在角膜碱烧伤后角膜新生血管(corneal neovascularization,CNV)形成中的作用及机制。方法 建立兔CNV动物模型,将其随机分为对照组和3个实验组,对照组结膜下注射生理盐水,实验组注射不同剂量的三氧化二砷。观察兔CNV的生长情况,并记录CNV的生长面积。用免疫组织化学方法检测兔角膜HIF、iNOS以及VEGF的表达。结果 对照组6 d、12 d、15 d、21 d、28 d CNV的面积分别为:(53.86±20.60)mm2、(87.21±25.80)mm2、(128.31±40.10)mm2、(114.42±29.40)mm2、(78.15±35.13)mm2。各实验组CNV面积均小于对照组,差异均有统计学意义(均为P<0.05)。免疫组织化学方法检测角膜HIF-1α、iNOS及VEGF蛋白的表达:VEGF表达集中在角膜上皮下基质,存在于血管内皮细胞、炎性细胞胞浆内,呈深黄色。在7 d有较高表达,14 d达到高峰,28 d时明显降低,且对照组明显高于实验组,各实验组的表达随三氧化二砷剂量的增加而减少。蛋白的表达与CNV面积呈正相关。结论 角膜碱烧伤后CNV的形成可能与HIF-1α有关。三氧化二砷通过抑制HIF-1α和iNOS、VEGF表达而抑制兔CNV的形成。
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| 关 键 词： | 缺氧诱导因子 血管内皮生长因子 一氧化氮合成酶 新生血管 三氧化二砷 |
Study of HIF on corneal neovascularization after corneal alkali burn in rabbits |
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| HU Qi,WANG Jian-Wen,CUI Mei-Zhi,WANG Ke-Meng,ZHOU Wen-Yan,XU Yang-Tao,LUO Jie,YU Jia-Wei From the Center of Optometry of the First Affiliated Hospital of Harbin Medical University,Harbin ,Heilongjiang Province,China.Study of HIF on corneal neovascularization after corneal alkali burn in rabbits[J].Recent Advances in Ophthalmology,2011(7):612-614. |
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| Authors: | HU Qi WANG Jian-Wen CUI Mei-Zhi WANG Ke-Meng ZHOU Wen-Yan XU Yang-Tao LUO Jie YU Jia-Wei From the Center of Optometry of the First Affiliated Hospital of Harbin Medical University Harbin Heilongjiang Province China |
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| Institution: | HU Qi,WANG Jian-Wen,CUI Mei-Zhi,WANG Ke-Meng,ZHOU Wen-Yan,XU Yang-Tao,LUO Jie,YU Jia-Wei From the Center of Optometry of the First Affiliated Hospital of Harbin Medical University,Harbin 150001,Heilongjiang Province,China |
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| Abstract: | Objective To investigate the effects and mechanisms of hypoxia inducible factor(HIF),inducible nitric oxide synthase(iNOS)and vascular endothelial growth factor(VEGF)in rabbit corneal neovascularization(CNV)after alkaline burn.Methods CNV models were established,and randomly divided into control group and three experimental groups.Rabbits in control group were treated with subconjunctival injection of normal saline;Rabbits in experimental groups were treated with different doses of arsenic trioxide.Growth c... |
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| Keywords: | hypoxia inducible factor vascular endothelial growth factor nitric oxide synthase neovascularization arsenic trioxide |
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