诱导子对黄芩毛状根生长及黄芩苷合成的影响

目的 考察诱导子对黄芩毛状根生长和黄芩苷生物合成的影响。方法 用非生物诱导子(Ca²⁺、Mg²⁺和Co²⁺)和从黑曲霉Aspergillus niger、米曲霉A. oryzae、蜜环菌Armillaria mellea中提取的真菌诱导子，与黄芩毛状根共培养；HPLC法测黄芩苷的量。结果 各诱导子对黄芩毛状根生长和黄芩苷合成有不同影响：黑曲霉诱导子和米曲霉诱导子质量浓度为40和20 mg/L时，Co²⁺浓度为1.53×10^-4 mmol/L时，黄芩苷的量从7.64%分别增至9.18%、8.81%和8.62%。结论 诱导子对黄芩毛状根次生代谢产物具有特异性，可选择性地促进某一类次生代谢产物的合成。试验证实黑曲霉诱导子、米曲霉诱导子和Co²⁺是适合黄芩毛状根代谢黄芩苷调控的诱导子。

Influence of elicitors on growth and baicalin biosynthesis in hairy root of Scutellaria baicalensis

Objective To study the influence of elicitors on growth and baicalin biosynthesis in hairy root of Scutellaria baicalensis (HRSB). Methods The elicitors from the solution of Ca²⁺, Mg²⁺, Co²⁺ and fungal elicitors extracted from Aspergillus niger, A. oryzae, and Armillaria mellea were cocultivated with HRSB and the content of baicalin was determined by HPLC. Results Every elicitor has different influences on the growth and baicalin biosynthesis. When the concentration of A. niger was 40 mg/L, A. oryzae was 20 mg/L, and the Co²⁺ is 1.53×10^-4 mmol/L, the content of baicalin increased respectively to 9.18%, 8.81%, and 8.62%. Conclusion Elicitors could improve some specific secondary metabolic synthesis. The test implies that the elicitors of A. niger, A. oryzae, and Co²⁺ could effectively improve the synthesis of baicalin.