| 砷对大鼠海马超微结构和NMDAR表达影响 |
| |
| 引用本文： | 罗教华,邱志群,舒为群,张勇燕,付文娟.砷对大鼠海马超微结构和NMDAR表达影响[J].中国公共卫生,2008,24(12). |
| |
| 作者姓名： | 罗教华 邱志群 舒为群 张勇燕 付文娟 |
| |
| 作者单位： | 第三军医大学军事预防医学院环境卫生教研室,重庆,400038 |
| |
| 基金项目： | 国家自然科学基金，重庆市自然科学基金 |
| |
| 摘 要： | 目的 观察饮水砷暴露后大鼠海马超微结构和N-甲基-D-天冬氨酸受体(NMDAR)亚基mRNA表达的变化,探讨砷致学习记忆损害的分子机制.方法 将48只雄性健康断乳SD大鼠随机分为4组,每组12只.对照组饮用自来水,砷暴露组分别饮用2.72,13.6和68 mg/L亚砷酸钠溶液.饲养3个月后.测定脑砷,用透射电镜观察海马超微结构,用RT-PCR检测海马组织NMDA受体亚基mRNA表达.结果 脑砷值随饮水砷浓度升高而升高,13.6和68 mg/L组与对照组相比,差异有统计学意义(P＜0.05).电镜下,染砷组大鼠海马神经细胞肿胀,线粒体肿胀.脊减少或无脊,粗面内质网扩张,血管内皮细胞肿胀.与对照组相比,各砷暴露组NR2A mRNA表达量明显降低(P＜0.05).结论 大鼠砷暴露可致海马神经细胞和血管内皮细胞的损伤,降低NR2A mRNA表达量,进而可能影响学习记忆功能.
|
| 关 键 词： | 砷 海马 超微结构 N-甲基-D-天冬氨酸受体 |
Effect of arsenic exposure from drinking water on NMDAR subunit mRNA expression and ultrastructural changes in hippocampus of rats |
| |
| LUO Jiao-hua,QIU Zhi-qun,SHU Wei-qun,et al..Effect of arsenic exposure from drinking water on NMDAR subunit mRNA expression and ultrastructural changes in hippocampus of rats[J].Chinese Journal of Public Health,2008,24(12). |
| |
| Authors: | LUO Jiao-hua QIU Zhi-qun SHU Wei-qun |
| |
| Institution: | LUO Jiao-hua,QIU Zhi-qun,SHU Wei-qun,et al.Department of Environmental Hygiene,College of Preventive Medicine,Third Military Medical University(Chongqing 400038,China) |
| |
| Abstract: | Objective To investigate NMDAR subunit mRNA expression and ultrastructural changes in hippocampus of rats after exposed to arsenic.Methods Weaned SD rats were divided into four groups randomly and each group had 12 rats.The control group drank tap water,the other three groups drank 2.72 mg/L,13.6 mg/L and 68 mg/L solution of sodium arsenite for 3 months.The arsenic levels of brain were assessed,the ultrastructual changes of rat hippocampus were observed with transmission electron microscope,and the expressi... |
| |
| Keywords: | arsenic hippocampus ultrastructure NMDAR |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
