胰酶中多种蛋白水解酶的分离和纯化 |
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引用本文: | 马建农,潘士皓,戚正武,周祖荫.胰酶中多种蛋白水解酶的分离和纯化[J].中国医药工业杂志,1988(9). |
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作者姓名: | 马建农 潘士皓 戚正武 周祖荫 |
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作者单位: | 上海生物化学研究所,上海生物化学研究所,上海生物化学研究所,上海科学技术大学 |
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摘 要: | 报道了一条综合利用胰酶的有效途径。对其中的羧肽酶、胰蛋白酶、糜蛋白酶等六种蛋白酶同时进行了分离和纯化。还对羧肽酶 A 和 B 的固定化条件及稳定性进行了探讨。
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关 键 词: | 猪胰蛋白水解酶 纯化 固定化 |
ISOLATION AND PURIFICATION OF SIX PROTEINASES FROM PORCINE PANCREAS |
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Authors: | MA Jian-Nong PAN Shi-Hao QI Zheng-Wu ZHOU Zu-Yina |
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Institution: | Shanghai Institute of Biochemistry;~aShanghai University of Science and Technology |
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Abstract: | This paper reports an effective procedure for comprehensiveutilization of porcine pancreas.Six proteinases(trypsin,chymotrypsin A,carboxypeptidase A,carboxypeptidase B,kallikrein and elastase)have beenconsecutively isolated and purified.The conditions for immobilization ofcarboxypeptidase A,B and stability of these immobilized enzymes have alsobeen studied. |
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Keywords: | porcine pancreatic proteinases purification immobilization |
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