| 反相高效液相色谱法测定蒲地蓝消炎片中黄芩苷的含量 |
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| 引用本文： | 席文胜,关小彬,张艳玲. 反相高效液相色谱法测定蒲地蓝消炎片中黄芩苷的含量[J]. 中国药房, 2006, 17(24): 1899-1900 |
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| 作者姓名： | 席文胜 关小彬 张艳玲 |
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| 作者单位： | 广东省中医院珠海医院药剂科,珠海市,519015 |
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| 摘 要： | 目的:建立以反相高效液相色谱法测定蒲地蓝消炎片中黄芩苷含量的方法。方法:色谱柱为AgilentC18,流动相为甲醇-0.1%磷酸(47∶53),检测波长为280nm,柱温为25℃,流速为1.0ml/min。结果:黄芩苷进样量在0.0576μg～0.864μg范围内与峰面积积分值线性关系良好(r=0.9999),平均加样回收率为99.14%(RSD=2.44%)。结论:本方法简便、准确、可靠、专属性强、重现性好,可用于本品的质量控制。
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| 关 键 词： | 反相高效液相色谱法 蒲地蓝消炎片 黄芩苷 含量 |
| 文章编号： | 1001-0408(2006)24-1899-02 |
| 收稿时间： | 2006-09-10 |
| 修稿时间： | 2006-10-08 |
Determination of Baicalin in Pudilanxiaoyan Tablets by RP- HPLC |
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| XI Wensheng,GUAN Xiaobin,ZHANG Yanling. Determination of Baicalin in Pudilanxiaoyan Tablets by RP- HPLC[J]. China Pharmacy, 2006, 17(24): 1899-1900 |
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| Authors: | XI Wensheng GUAN Xiaobin ZHANG Yanling |
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| Affiliation: | Dept .of Pharmacy, Zhuhai Hospital, Guangdong Hospital of TCM, Zhuhai 519015, China |
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| Abstract: | OBJECTIVE:To establish a RP-HPLC method for the determination of baicalin in Pudilanxiaoyan Tablets.METHODS:The separation was performed on Agilent ODS C18 with methaol-0.1% H3PO4(47∶ 53)as mobile phase.The dete_ctive wavelength was set at 280nm;the column temperature was 25℃,and the flow rate was 1.0ml/min.RESULTS:Baicalin showed a good linearity in the range of 0.0 576μ g～ 0.864μ g(r=0.9 999).The average recovery was 99.14%(RSD=2.44%).CONCLUSION:The method is simple,accurate,reliable,specific and reproducible,and suitable for the quality control of Pudilanxiaoyan tablets. |
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| Keywords: | RP-HPLC Pudilanxiaoyan Tablets Baicalin Content |
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