PTEN和p53蛋白在肝癌组织中的表达及意义

背景与目的：抑癌基因PTEN于1997年首次被报道之后即成为研究热点。国外曾有研究小组检测人类肝癌中PTEN基因突变情况,但对有关PTEN蛋白在肝癌中的表达情况了解甚少;p53基因突变是肝癌发生的机制之一,但对于p53基因突变与肝癌某些生物学行为的相关性,不同研究的结果不一。我们拟通过检测PTEN、p53蛋白在肝癌中的表达来了解PTEN、p53与肝癌发生和进展的关系。方法：本研究共取62例石蜡包埋的肝癌标本,所有组织切片均含肝癌组织及癌旁组织。采用免疫组化SP法检测PTEN及突变的p53蛋白的表达情况。结果：62例标本中癌旁肝组织均呈现明确的PTEN阳性染色（胞浆）,只有29例（46.8％）标本中肝癌组织呈阳性染色,PTEN在有包膜或无侵袭性肝癌中的表达明显高于无包膜或有侵袭性的肝癌。所有标本癌旁肝组织均未见p53阳性染色,21例肝癌组织p53染色呈阳性（胞核）。P53蛋白的表达与肝癌侵袭性或分化程度无关。结论：PTEN的失表达及p53基因突变可能在肝癌发生中发挥作用,PTEN失表达还可能与肝癌的恶性进展有关。

Expression of phosphatase and tensin homology deleted on chromosome ten(PTEN) and p53 protein and their significance in human hepatocellular carcinomas

BACKGROUND&OBJECTIVE:Tumor suppressor g ene phospha-tase and tensin homolog y deleted on c hromosome ten(PTEN)was first reported in 1997and has become a hot spot shortly after discovery.Mutations of PTE N g ene in human hepatocellular carcin omas (HCCs )have been examined by several g roups abroad,while little is known about expression of PTEN in HCCs.It has been believed that p 53mutatio n plays a role in the oncog enesis of HCCs .However,diverg ence exist as r eg ards the relationship between p 53mutation and some biolog ical behavi ors of HCCs .The current study was desig ned to investig ate the correla tions of expression of PTEN and mutan t p53with carcinog enesis and tumor prog r ession of HCCs.METHODS :Sixty-two paraffin-embedded hepatocellular carcinoma samples were composed and all the tissue sections included both HC C and surrounding non-tumor liver tissues .Expression of PTEN and muta nt p53was examined using SP immunohistochemical technique.RESULTS :All sixty-two samples showed decidedly positive staining of PTEN in surrounding non-tumor liver tiss ues (plasma ).While,only twenty-nine tumors wer e detected positive staining of PTEN(46.8%).The expression level of PTEN in HCC s amples without invasiveness or with complete capsu le was hig her than that in HCC samples with invasiveness or without complete ca psule.No positive staining of p53wa s detected in surrounding non-tumor l iver tissues for all sixty-two sampl es.However,twenty-one tumors showed p ositive staining of p53(nucleon).The expression level of p53protein was n ot related with invasion or differen tiation of HCCs.CONCLUSION:Loss of PTEN expression and mutation of p53may play a role in occurrence of HCCs.Loss of PTEN expression may be pertinent to deterioration of HCCs.