双参素胶囊对大鼠实验性心肌缺血再灌注损伤的保护作用

 目的 观察双参素胶囊对大鼠实验性心肌缺血再灌注损伤的保护作用.方法 通过在体大鼠结扎左冠状动脉前降支30min,再灌注24 h制备实验性心肌缺血再灌注损伤模型,双参素胶囊按17.5mg/kg、35 mg/kg、70 mg/kg给大鼠连续灌胃7 d,阳性药物组连续灌胃福辛普利20 mg/kg,7 d,假手术组及再灌模型组灌胃0.5%羧甲基纤维素钠,计算心肌梗死范围(MIS),测定血清天冬酸氨基转移酶(AST)等的活性.结果　双参素胶囊可使实验性心肌缺血再灌注损伤大鼠的MIS明显缩小(P＜0.05或P＜0.01),血清AST、LDH、CK-MB活性及MDA含量明显降低(P＜0.05或P＜0.01),血清SOD及GSH-Px活性明显增加(P＜0.05或P＜0.01),亦可使血浆ET和AngⅡ含量明显下降(P＜0.05或P＜0.01).结论 双参素胶囊对大鼠实验性心肌缺血再灌注损伤具有明显保护作用.

Protective effects of Shuangshensu capsules on experimental myocardial ischemia-reperfusion injury in rats

Objective To study the protective effects of Shuangshensu capsules （SSC） on experimental myocardial ischemia - repeffu- sion injury in rats.Methods The rat myocardial ischemiareperfusion model was developed by 30 min left anterior descending coronary occulusion and 24 h reperfusion in open chest anesthetized rats. The activities of aspartate aminotransferase （AST） and other Myocardial enzymes were determined. Results In rats treated with SSC （35 and 70 mg/kg i. g for 7 d）, the myocardial ischemia size was significantly reduced （ P 〈 0.05 or P 〈 0.01）, the AST, LDH and CK - MB activities and the MDA content in serum declined （ P 〈 0.05 or P 〈 0.01 ）, while the SOD and GSH- Px activities in serum increased significantly （ P 〈 0.05 or P 〈 0.01）. In addition,the ET and Magl] levels in plasma also declined markedly. Conclusion SSC have protective effects on myocardial ischemia - reperfusion injury.