| 改良酸测琼脂板法检测金黄色葡萄球菌的青霉素酶 |
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| 引用本文： | 陈惠青. 改良酸测琼脂板法检测金黄色葡萄球菌的青霉素酶[J]. 中南大学学报(医学版), 1990, 0(2) |
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| 作者姓名： | 陈惠青 |
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| 作者单位： | 湖南医科大学微生物学教研室 |
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| 摘 要： | 用改良酸测琼脂板法检测了71株金黄色葡萄球菌的青霉素酶,并与Park酸测琼脂板法作了对比。结果二者完全一致,但改良法结果更为清晰易辨。与液体倍比稀释法所测最小抑菌浓度(MIC)结果也完全相符。实验证实本法为一准确、可靠、价廉、快速的检测细菌青霉素酶的方法。
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| 关 键 词： | 青霉素酶类 青霉素抗药性 金黄色葡萄球菌 分离和提纯 方法 |
MODIFIED ACIDOMETRIC AGAR PLATE METHOD FOR DETECTION OF PENICILLINASE OF STAPHYLOCOCCUS AUREUS |
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| Chen Huiqing. MODIFIED ACIDOMETRIC AGAR PLATE METHOD FOR DETECTION OF PENICILLINASE OF STAPHYLOCOCCUS AUREUS[J]. Journal of Central South University. Medical sciences, 1990, 0(2) |
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| Authors: | Chen Huiqing |
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| Affiliation: | Chen HuiqingDepartment of Microbiology,Hunan Medical University |
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| Abstract: | Since the resistance of Staphylococcus aureus to penicillin was found to be due to the production of penicillin beta-lactamase (penicillinase). We have employed modified acidomtric agar plate method to detect penicillinase of strains of S. aureus and contrasted with Park's acidometric agar plate method. The results of this two methods were identical, 58 strains were positive and 13 were negative, but the results of the modified method were clearer than that of Park's method. All results correlated with MICs. The results of experiment have been demonstrated that the modified acidometric agar plate method is a simple, inexpensive, accurate and rapid way tb determine S. aureus susceptibility of penicilli n. |
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| Keywords: | penicillinase penicillin resistance Staphylococcus aureus isolation and purification methods |
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