Lyn激酶在伊马替尼耐药的慢性髓系白血病中的作用

本研究探讨Lyn激酶在伊马替尼耐药的慢性髓系性白血病（CML）中的作用。76例CML患者分为初治组、伊马替尼耐药组和伊马替尼治疗有效组，用Western blot方法检测CML患者骨髓单个核细胞中Lyn蛋白的表达水平，比较各组患者Lyn的表达差异，分析Lyn与I临床特征、BCR／ABL融合基因和染色体的关系。结果表明，76例CML均表达Lyn；伊马替尼耐药组Lyn表达明显高于正常对照组、初治组及伊马替尼治疗有效组（P〈0．05），初治组和伊马替尼治疗有效组的Lyn表达水平与正常对照组无明显差别（P〉0．05）。Lyn表达水平与性别、年龄、平均血红蛋白水平、平均血小板计数、外周血幼稚细胞比例和脾脏大小无明显相关（P〉0．05），但与初诊时外周血白细胞计数增高相关（P〈0．05）。Lyn表达与BCR／ABL融合基因定量无明显相关性（P〉0．05）；10例伊马替尼耐药的CML中，1例患者存在t（6；22）和t（2；9）改变，与Ph染色体共存，其余9例患者仅存在Ph染色体改变。结论：CML患者和正常人骨髓细胞均表达Lyn，Lyn在伊马替尼耐药的CML中表达明显增高，Lyn高表达与CML患者初诊时白细胞计数明显增高相关。

Role of Lyn Kinase in Imatinib-resistant Chronic Myelogenous Leukemia

This study was aimed to explore the role of Lyn kinase in imatinib-resistant CML, Lyn, BCR/ABL fusion gene and chromosomes were detected in 76 CML patients being divided into imatinib-resistant, newly diagnosed and effective groups, and then the expression of Lyn was compared and the relationship between Lyn and clinical characteristics, BCR/ABL fusion gene and chromosomes were analyzed. The results indicated that all the 76 CML patients and 10 normal persons expressed Lyn. Lyn expression in imatinib-resistant patients was significantly higher than that in normal persons, newly diagnosed patients and imatinib-effective patients （P 〈 0.05 ）. However, there was no statistically significant difference between newly diagnosed patients, effective patients and normal persons （ P 〉 0.05 ）. Lyn expression had no significant correlation with median age, sex, median hemoglobin level, and median platelet level, percentage of peripheral blasts, spleen size （ P 〉 0. 05 ）. The Lyn expression was related with the higher count of peripheral blood leukocytes at new diagnosis （ P 〈 0.05 ）. There was no obvious relationship between Lyn and BCR/ABL levels （ P 〉 0.05 ）. There was 1 case with chromosome abnormality in t （6 ; 22 ） and t （2 ; 9 ） in 10 imatinib-resistant CML patients, coexisting with Ph chromosome. Ph chromosome only existed in the remanent 9 cases of CML. It is concluded that both the CML patients and normal persons express Lyn. Lyn is over-expressed in imatinib-resistant CML. The increased Lyn expression is closely related with higher WBC count.