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| 抗氧化能力指数(ORAC)测定原理及应用 | |
| 引用本文： | 续洁琨,姚新生,栗原博.抗氧化能力指数(ORAC)测定原理及应用[J].中国药理学通报,2006,22(8):1015-1021. |
| 作者姓名： | 续洁琨 姚新生 栗原博 |
| 作者单位： | 1. 沈阳药科大学中药学院,辽宁,沈阳,110016 2. 沈阳药科大学中药学院,辽宁,沈阳,110016;暨南大学药学院,广东,广州,510632 3. 暨南大学药学院,广东,广州,510632 |
| 摘 要： | 目的介绍抗氧化能力指数测定方法的原理、应用及发展前景。方法被译为抗氧化能力指数的oxygenradicalabsorbancecapacity(ORAC)方法,是目前抗氧化研究领域中为人们所关注的一个评价方法。该方法以偶氮类化合物AAPH作为过氧自由基来源,SodiumFluorescein为荧光指示剂,维生素E水溶性类似物Trolox为定量标准,使用荧光微孔板分析仪进行分析。结果方法的专属性、线性、精密度、准确度及重复性等与其他抗氧化能力分析方法相比具有诸多显著的优点。结论该方法目前已成功应用于生物样品、植物或食品提取物和纯化合物等多种样品的体内外抗氧化能力分析,并有希望在研究氧化与疾病发生关系等方面发挥重要的作用。 |
| 关 键 词： | 抗氧化能力指数 自由基 AAPH sodium fluorescein trolox |
| 文章编号： | 1001-1978（2006）08-1015-07 |
| 收稿时间： | 2006-04-05 |
| 修稿时间： | 2006-04-052006-06-21 |
Oxygen radical absorbance capacity assay and its application |
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| XU Jie-kun,YAO Xin-sheng,Hiroshi Kurihara.Oxygen radical absorbance capacity assay and its application[J].Chinese Pharmacological Bulletin,2006,22(8):1015-1021. | |
| Authors: | XU Jie-kun YAO Xin-sheng Hiroshi Kurihara |
| Institution: | 1. School of Traditional Chinese Materia Medica , Shenyang Pharmaceutical University, Shenyang 110016, China ; 2. Pharmacy College of Jinan University, Guangzhou 510632, China |
| Abstract: | Aim To summarize the principles, applications and future development in oxygen radical absorbance capacity (ORAC) assay. Methods The ORAC assay method use Sodium Fluorescein as fluorescent probe, AAPH as free radical initiator, and results are expressed as Trolox equivalents. Results This method has several advantages compared with other methods in linear responses with concentration, specificity, precision, accuracy and ruggedness. Conclusion The ORAC assay has been widely accepted and largely applied to the assessment of free radical scavenging capacity of pure compounds, biological samples, plant and food extracts. |
| Keywords: | AAPH sodium fluorescein trolox |
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