不同剂量孕马血清促性腺激素(PMSG)对未成年大鼠超数排卵效果与质量的影响

目的：建立SD大鼠的有效超数排卵程序，为大鼠胚胎生物工程相关技术的研究提供参考。方法：分别用10、20、30、40、50IU的孕马血清促性腺激素/人绒毛膜促性腺激素（PMSG／hCG）对4～5周龄、6～7周龄、8～9周龄SD大鼠进行超数排卵，比较超排效果，并将超排处理后体内受精的2-细胞胚胎移植给受体雌鼠，观察妊娠产仔情况。结果：4～5周龄组SD大鼠在激素剂量为20IU／只时，平均超排卵54．6枚，明显高于其他组合；6～7周龄组在激素剂量为40IU／只时超排卵数最多，为32．7枚，但与30IU和50IU剂量组差异不显著；8～9周龄组超排效果最差，平均超排卵20枚左右。超排处理的2-细胞胚胎移植给代孕鼠后，妊娠率为63．3％，与对照组（60％）差异不显著。结论：SD大鼠的超排效果随着年龄增长而下降，4～5周龄雌鼠超排效果最好，其中激素剂量为20IU／只时超排卵数最多，且超排的卵子质量正常，受精后可以完成全程发育。

Effects of the PMSG Dosages on Superovulation and Oocyte Quality in Immature Rats

Objective To establish an effective method for superovulatory treatment of rats with pregnant mare serum gonadotrophin(PMSG) and human chorionic gonadotropin(hCG). Methods Sprague-Dawley rats at different age(4-5 weeks,67 weeks and 8-9 weeks) were superovulated with PMSG and hCG at a dosage of 10 IU, 20 IU,30 IU,40 IU or 50 IU,respectively.The results of superovulation of those rats were analyzed.Finally,the 2-cell embryos fertilized in vivo were transferred to recipients to observe the rate of pregnancy.Results Rats aged 4-5 weeks showed the best effect of PMSG/hCG at a dosage of 20 IU on superovulation,with an average number of 54.6 per rat,while rats aged 6-7 weeks and 8-9 weeks were 32.7 and 21.5 per rat,respectively.After embryo transfer,the rate of pregnancy was 63.3%,showing no significant difference with that of control rats(60%).Conclusion Rats at 4-5 weeks of age are more sensitive to superovulation,giving a highest number of oocytes with 20 IU PMSG and hCG.Such oocytes obtained by superovulation are good in quality and can complete the whole term development after fertilized in vivo and embryo transfer.