益母草水苏碱对肥大心肌细胞活性氧信号的影响

目的:观察益母草水苏碱对心肌细胞肥大的作用,探讨其对活性氧(ROS)及其下游NF-κB信号的影响。方法:原代培养乳鼠的心肌细胞,用血管紧张素Ⅱ建立细胞肥大模型,观察10-7～10-4mol/L浓度的益母草水苏碱对细胞肥大的影响(测定细胞体表面积及Protein/DNA比值),同时利用流式细胞仪检测ROS阳性细胞比例,West-ern Blot法测定细胞浆内p-IκBα(ser32)及核内NF-κB(p65)蛋白表达。结果:一定浓度的益母草水苏碱可以降低肥大心肌细胞的体表面积、Protein/DNA比值以及ROS阳性细胞比例(P0.05);益母草水苏碱干预降低了肥大心肌细胞浆内p-IκBα(ser32)及核内NF-κB(p65)蛋白的表达水平(P0.05)。结论:对ROS及其下游NF-κB信号的抑制可能涉及益母草水苏碱对抗心肌细胞肥大的作用机制。

Effect of Leonurus Stachydrine on Myocardial Cell Hypertrophy

Objective: To investigate the effects of Leonurus stachydrine on myocardial cell hypertrophy induced by angiotensin II in rats.Methods: Myocardial cells of neonatal rats were incubated with angiotensin II for 48 hours to establish myocardial cell hypertrophy models.The cell surface area and protein/DNA ratio were detected as index for hypertrophy of myocardial cells.ROS positive myocardial cell were counted by means of flow cytometry,the expression of p-IκB(ser32)protein in the cytosol and NF-κB(p65) protein in the nucleus were also detected by Western blot.Results: Various concentrations of stachydrine intervention(10-6-10-4mol/L) showed better anti-hypertrophic effect and decreased the number of ROS positive myocardial cell(P<0.05).Meanwhile,intervention of stachydrine significantly suppressed the level of p-IκB(ser32)protein in the cytosol and NF-κB(p65) protein in the nucleus(P<0.05).Conclusion: Stachydrine can inhibit the NF-κB signal pathway,and this may be ralated to the mechanism of anti-hypertrophic.