| 骨形成蛋白-2对室管膜前下区神经干细胞DLX5表达的影响 |
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| 引用本文： | 刘胜华,周政,刘俊,阴金波.骨形成蛋白-2对室管膜前下区神经干细胞DLX5表达的影响[J].华西医学,2010(12):2177-2180. |
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| 作者姓名： | 刘胜华 周政 刘俊 阴金波 |
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| 作者单位： | 第三军医大学新桥医院神经外科,重庆400037 |
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| 基金项目： | 全军“十一五”计划课题（06MB236） |
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| 摘 要： | 目的 探讨骨形成蛋白-2（bone morphogenetic protein-2，BMP-2）对室管膜前下区（anterior subventricular zone，SVZa）神经干细胞DLX5表达的影响。方法体外培养SVZa神经干细胞，用BMP-2及其拮抗剂Noggin诱导SVZa神经干细胞，分别用免疫荧光染色和逆转录一聚合酶链反应（RT-PCR）检测DLX5表达变化。结果BMP-2组SVZa神经干细胞DLX5蛋白表达和DLX5mRNA表达水平明显高于对照组（P〈0．05），且该效应能被其拮抗剂Noggin特异性地抑制。结论BMP-2是DLX5上游调节基因，可促进SVZa神经干细胞DLX5的表达。
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| 关 键 词： | 神经干细胞 室管膜前下区 DLX5 骨形成蛋白-2 Noggin |
Effects of Bone Morphogenetic Protein-2 on Expression of DLX5 of the Neural Stem Cells of Anterior Subventricular Zone |
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| LIU Sheng-hua,ZHOU Zheng,LIU J un,YIN Jin-bo.Effects of Bone Morphogenetic Protein-2 on Expression of DLX5 of the Neural Stem Cells of Anterior Subventricular Zone[J].West China Medical Journal,2010(12):2177-2180. |
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| Authors: | LIU Sheng-hua ZHOU Zheng LIU J un YIN Jin-bo |
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| Institution: | . (Department of Neurosurgery ,Xinqiao Hospital,the Third Millitary Medical University ,Chongqing 400037,P. R. China) |
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| Abstract: | Objective To investigate the effect of bone morphogenetic protein 2 （BMP 2）on expression of DLX5 of neural stem cells in anterior subventricular zone （SVZa）. Methods The neural stem cells of SVZa were separated and cultured in vitro, which were induced by BMP2 and Noggin. Immunofluorescence staining and RT-PCR were employed to assay the expression of DLX5. Results The percentages of expression of DLX5 protein and DLX5 mRNA in BMP-2 group were much higher than those in the control group （P〈0. 05）. And this induction could be specifically blocked by Noggin. Conclusion BMP-2 is an upstream gene of DLX5 ; BMP-2 can promote the expression of DLX5 of the neural stem cells of SVZa. |
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| Keywords: | Neural stem cells Subventricular zone DLX5 Bone morphogenetic protein-2 Noggin |
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