体外受精后提前去除卵丘细胞对临床结局的影响

目的观察提前去除卵丘细胞对胚胎质量的影响,进一步探讨其临床应用价值。方法将2009年79月行体外受精胚胎移植治疗的患者随机分为两组,试验组共137个周期,于授精后5h去除卵丘细胞;对照组共146个周期,于授精后16～18h去除卵丘细胞。两组均于授精后16～18h观察受精情况,72h对胚胎进行评分,选择1～3枚优质胚胎进行胚胎移植。分别比较两组的年龄、不育年限、获卵数、受精率、卵裂率、优质胚胎率、胚胎种植率和临床妊娠率。结果两组的优质胚胎率差异有统计学意义（P〈0.01）;但年龄、不育年限、获卵数、受精率、卵裂率、胚胎种植率和临床妊娠率比较,差异均无统计学意义（P〉0.05）。结论体外受精中提前去除卵丘细胞优质胚胎增加,有助于提高临床累积妊娠率。

Effect of Denuding the Oocyte Granulose Cells Soon After Fertilization in Vitro

Objective To evaluate the effect of denuding the oocyte granulose cells soon after fertilization in vitro（IVF）.Methods A total of 283IVF cycles in patients from July to September 2009were divided into a trial group and a control group randomly.The trial group contained 137cycles,being denuded the oocyte granulose cells only 5hours after IVF.The controlled group contained 146cycles,being denuded the oocyte granulose cell and observed the pronuclear 18hours after IVF.The outcomes of female age,infertile duration,the number of oocyte,fertilization rate,abnormal fertilization rate,good quality embryo rate,and clinical pregnancy rate were measured.Results There was no difference in any of the outcomes between the two groups except for the high quality embryo rate（P 0.01）.The clinical pregnancy rate was higher in the trial group without significant differences（P0.05）.Conclusions Denuding the oocyte granulose cells soon after IVF helps to improve the embryo quality and the clinical accumulated pregnancy rate.