黄精多糖对老龄大鼠老化相关酶的影响

目的观察黄精多糖（polygonatum sibiricmu polysaccharides,PSP）对老龄大鼠血液中老化相关酶的影响。方法取老龄（15～16月龄）SD大鼠。每组15只,均采用灌胃给药。阴性对照组灌服等体积的0.5%羧甲基纤维素（CMCNa）;阳性对照组给予甲磺酸双氢麦角碱0.5mg/kg;PSP大剂量组222mg/（kg·d）;PSP中剂量组111mg/（kg·d）;PSP小剂量组55mg/（kg·d）;另取9只青年SD大鼠,作为青年对照组,灌服等体积的0.5%CMCNa。每日1次,连续4周,末次给药后取血,检测老化相关酶：超氧化物歧化酶（SOD）、过氧化脂质（LPO）、脂褐质（LF）、B型单胺氧化酶（MAO-B）。各项指标进行统计分析,比较各组差异。结果 PSP高、中剂量组的SOD水平明显提高,而LPO、LF和MAO-B水平明显下降。结论 PSP有较明确的延缓衰老作用。

Effect of Polygonatum Sibiricmu Polysaccharides on Enzymes Related to the Aging of Aged Rats

Objective To observe the effects of Polygonatum sibiricmu polysaccharides （PSP） on enzymes related to the aging in the blood of aged rats.Methods Seventy-five aged rats （15-16 months） were divided into 5 groups randomly containing 15 rats in each group.The rats of each group were treated with different medicines daily for 4 weeks by gavage：negative control group received 0.5% CMCNa; positive control group received dihydroer gotoxine of 0.5 mg/kg; high dose group of PSP received PSP of 222 mg/（kg·d）; middle dose group of PSP received PSP of 111 mg/（kg·d）; low dose group of PSP received PSP of 55 mg/（kg·d）.Another 9 young rats （4 male and 5 female rats） were chosn as an additional control group to study the reaction of youth,which received 0.5% CMCNa by gavage.The enzymes about aging were detected after the last treatment,such as superoxide dismutase （SOD）,lactoperoxidase （LPO）,lipofuscin （LF）,monoamine oxidase type B （MAO-B）.Statistical analysis was used to judge the differences of all groups.Results The blood level of SOD in high and middle dose groups of PSP were significantly increased while the blood level of LPO,LF,MAO-B were decreased with markable differences.Conclusion PSP has an obvious effect of anti-aging.