妊娠肝内胆汁淤积症患者血清对体外培养的胎盘组织促肾上腺皮质激素释放激素分泌水平的影响

目的观察妊娠肝内胆汁淤积症（intrahepatic cholestasis of pregnancy,ICP）患者血清对体外培养的胎盘组织促肾上腺皮质激素释放激素（corticotropin-releasing hormone,CRH）的分泌水平的影响。方法收集2005年3月7月在四川大学华西第二医院产科住院分娩的正常妊娠妇女胎盘组织及其血清（对照组）与ICP患者血清（ICP组）各10例。通过胎盘组织培养及放射免疫法测定其培养液中CRH水平。结果 ICP组胎盘组织CRH分泌水平低于对照组,ICP组24、48、72、96h分别为（84.95±34.98）、（74.57±29.93）、（71.16±27.26）、（81.07±37.18）pg/mL;对照组分别为（103.74±30.85）、（108.27±23.77）、（109.20±23.81）、（118.15±26.84）pg/mL。两组比较,48h后差异有统计学意义（P〈0.05）。结论 ICP患者血清对体外培养的胎盘组织CRH分泌有抑制作用。

Effect of Serum of Pregnant patients with Intrahepatic Cholestasis on the Excretion of Corticotropin-Releasing Hormone in the Placental Tissue in Vitro

Objective To observe the effect of serum of pregnant patients with intrahepatic cholestasis（ICP）on the excretion level of corticotrophin-releasing hormone in the placental tissue in vitro.Methods Serum from 10patients with ICP（ICP group）and from the healthy placental tissue of 10normal people（control group）were collected from March to July,2005.Cell culture and radioimmunoassay methods were used to investigate the corticotropin-releasing hormone（CRH）levels in placental tissue.Results The level of CRH in human placental tissue treated with sera of ICP was lower than that in the control group.24,48,72,and 96hours after treated with the serum,the levels of CRH in the ICP group were（84.95±34.98）,（74.57±29.93）,（71.16±27.26）,and（81.07±37.18）pg/mL,respetively;while in the control group were（103.74±30.85）,（108.27±23.77）,（109.20±23.81）,and（118.15± 26.84）pg/mL,respectively.There was significant difference in the levels of CRH between ICP group and control group 48hours after the culture（P0.05）.Conclusion The serum from the patients with ICP may inhibit the excretion of CRH in the placental tissue.