| 金刚石涂膜材料对成骨细胞骨钙素表达的影响 |
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| 引用本文： | 李增健,孔庆华,王玉新.金刚石涂膜材料对成骨细胞骨钙素表达的影响[J].中国医科大学学报,2005,34(4):326-327. |
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| 作者姓名： | 李增健 孔庆华 王玉新 |
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| 作者单位： | 1. 中国医科大学附属口腔医院颌面外科,辽宁,沈阳,110002
2. 沈阳市新兴齿科 |
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| 摘 要： | 目的:探讨不同种植材料表面成骨细胞的功能状态.方法:用Wistar大鼠颅顶骨建立体外培养成骨细胞模型,将第三代成骨细胞接种到金刚石涂膜(CVD)、羟基磷灰石(HA)、纯钛(Ti)3种种植材料表面,通过反转录聚合酶链式扩增方法测定成骨细胞骨钙素mRNA.结果:CVD表面的成骨细胞骨钙素mRNA表达比HA出现早,Ti表面未见骨钙素mRNA表达.结论:CVD表面骨钙素的早期表达,推测其有成骨功能,适合成骨细胞生长.
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| 关 键 词： | 金刚石涂膜 成骨细胞 反转录聚合酶链式扩增 骨钙素mRNA |
| 文章编号： | 0258-4646(2005)04-0326-02 |
| 收稿时间： | 2005-02-28 |
| 修稿时间： | 2005年2月28日 |
Effect of the chemical-vapor-deposited diamond film materials on expression of osteocalcin mRNA of osteoblast |
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| LI Zeng-jian,KONG Qing-hua,WANG Yu-xin.Effect of the chemical-vapor-deposited diamond film materials on expression of osteocalcin mRNA of osteoblast[J].Journal of China Medical University,2005,34(4):326-327. |
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| Authors: | LI Zeng-jian KONG Qing-hua WANG Yu-xin |
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| Abstract: | Objective: To evaluate the cell function of osteoblasts on the surface of different implant denture materials. Methods: The cultured osteoblastic cell model in vitro was made with calvaria of new born Wistar rats. The third generation osteoblasts were plated on the surfaces of 3 kinds of implant materials of chemical-vapor-deposited diamond film(CVD), hydroxyapatite, and pure titanium. The expressions of osteocalcin mRNA were examined by using RT-PCR. Results: The osteocalcin mRNA expression of CVD material group was earlier than that of hydroxyapatite group, and there was no expression in pure titanium group. Conclusion: CVD materials are fit for growth of osteoblasts, and it could promote the formation of the new bone. |
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