血啉甲醚体外光敏反应机制研究

目的：探讨血啉甲醚（HMME）及血卟啉衍生物（HpD)体外光敏化反应的机制。方法：采用HMME及HpD光敏引起还原型辅酶I（NADH）氧化为中介的鲁米诺(luminol)化学发光法（CL），探讨HMME及HpD光敏反应特点，选择性应用单态氮（^1O2)及活性氧物质（ROS）的专一清除剂，研究^1O2及RSO在HME及HpD光敏反应中的作用。结果:^1O2专一清除剂几乎完全抑制了HMME和HpD的化学发光，ROS清除剂对化学发光没有明显影响。HMME化学发光值最高可达509595mV，反应持续时间48s,^1O2的总产量12230280。HpD的化学发光值最高49000mV，反应时间235s,^1O2的总产量5757500。结论：HMME及HpD的光敏化产物主要是^1O2，HMME光敏化产生^1O2的光量子产率高于HpD，^1O2生成速度比HpD快，HMME自敏光氧化速度也比HpD快。在鲜红斑痣的的治疗中，当血供正常时，HMME光敏损伤作用可能更强，组织选择性可能更好。

Mechanism of Photosensitized Reaction Induced by Hematoporphyrin Monomethyl Ether in vitro

Objective To investigate the mechanism and characteristic of photosensitized reaction induced by hematoporphyrin monomethyl ether (HMME) and hematoporphyrin derivative (HpD) in vitro. Methods The mechanism and characteristic of photosensitized reaction and the effect of scavengers of 1 O 2 and ROS were assessed using luminol chemiluminescence induced by HMME and HpD. Results Scavengers of 1 O 2 almost completely inhibited chemiluminescence induced by HMME and HpD. However, scavengers of ROS had no effect on chemiluminescence. The highest volume of chemiluminescence and the total output of 1 O 2 induced by HMME in 48 second were 509 595 mV and 12 230 280, respectively while the highest volume of chemiluminescence and the total output of 1 O 2 induced by HpD in 235 second were 49 000 mV and 5 757 500, respectively. Conclusions In vitro, the main outcome during photosensitized reaction induced by HMME and HpD was 1 O 2. 1 O 2 Quantum yield induced by HMME was apparently higher than that of HpD. HMME, rate of producing 1 O 2 and photobleaching were much more rapid than that of HpD. When blood supply is normal, the killing effect of photosensitized reaction induced by HMME could be possibly stronge and HMME PDT, tissue selectivity could be probably good in the treatment.