| HPLC测定千柏鼻炎片中对羟基桂皮酸和金丝桃苷的含量 |
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| 引用本文： | 谭道鹏,侴桂新,,王峥涛,.HPLC测定千柏鼻炎片中对羟基桂皮酸和金丝桃苷的含量[J].中国现代应用药学,2010,27(6):532-534. |
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| 作者姓名： | 谭道鹏 侴桂新 王峥涛 |
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| 作者单位： | 1.中国药科大学中药学院，南京 210038；2.上海中医药大学中药研究所；中药标准化教育部重点实验室；中药新资源与质量标准综合评价国家中医药管理局重点研究室；上海市复方中药重点实验室，上海 201203；3.上海中药标准化研究中心，上海 201203 |
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| 基金项目： | “十一五”国家科技支撑计划（2006BAI14B01） |
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| 摘 要： | 目的建立千柏鼻炎片中对羟基桂皮酸和金丝桃苷的含量测定方法。方法应用高效液相色谱法测定,色谱柱为Dikma Diamonsil-C18（4.6mm×250mm,5μm）;流动相为乙腈-0.2%冰醋酸（15∶85）;流速1.0mL·min^-1;检测波长310nm（0～25min）,360nm（25～35min）。结果对羟基桂皮酸和金丝桃苷的线性范围分别为0.014088～1.174μg（r=0.9999）,0.00955～1.91μg（r=0.9999）;平均加样回收率分别为101.17%（RSD=1.35%）,100.89%（RSD=3.80%）。结论该方法简便易行、准确、重复性好,可用于千柏鼻炎片的质量控制。
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| 关 键 词： | 千柏鼻炎片 千里光 对羟基桂皮酸 金丝桃苷 |
Simultaneous Determination of p-Hydroxycinnamic Acid and Hyperoside in Qianbai Biyan Tablets by HPLC |
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| TAN Daopeng,CHOU Guixin,,WANG Zhengtao,.Simultaneous Determination of p-Hydroxycinnamic Acid and Hyperoside in Qianbai Biyan Tablets by HPLC[J].The Chinese Journal of Modern Applied Pharmacy,2010,27(6):532-534. |
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| Authors: | TAN Daopeng CHOU Guixin WANG Zhengtao |
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| Institution: | 1.College of Traditional Chinese Medicine China Pharmaceutical University, Nanjing 210038, China; 2.The MOE Key Laboratory for Standardization of Chinese Medicines and the SATCM Key Laboratory for New Resources and Quality Evaluation of Chinese Medicines; Institute of Chinese Material Medica, Shanghai University of Traditional Chinese Medicines, Shanghai 201203, China; 3.Shanghai R&D Center for Standardization of Chinese Medicines, Shanghai 201203, China |
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| Abstract: | OBJECTIVE To establish a high performance liquid chromatographic method for the determination of p-hydroxycinnamic acid and hyperoside in Qianbai Biyan tablets.METHODS The chromatographic separation was achieved on a Dikma Diamonsil-C18（250 mm×4.6 mm,5 μm） column at a variable wavelength from 310 nm（0-25 min） to 360 nm（25-35 min） using an isocratic mobile phase consisted of a mixture of 0.2% aqueous acetic acid and acetonitrile in the ratio of 85：15 at a flow rate of 1.0 mL·min^-1.RESULTS Linearity relationships were 0.014 088-1.174 μg（r=0.999 9）,0.009 55-1.91 μg（r=0.999 9）,respectively;the average recoveries were 101.17%（RSD=1.35%） and 100.89%（RSD=3.80%）.CONCLUSION The results showed that the method is simple,accurate and repeatable and it is suitable for the determination of p-hydroxycinnamic acid and hyperoside in Qianbai Biyan tablets. |
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| Keywords: | Qianbai Biyan tablets herba Senecionis Scandentis p-hydroxycinnamic acid hyperoside |
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