丁酸钠对人脑胶质瘤细胞系SHG-44增殖分化的影响

目的 研究丁酸钠对培养人脑胶质瘤SHG-44细胞的增殖抑制和分化诱导。方法 以2mmol／L丁酸钠处理SHG-44细胞，用MTT比色、核仁组成区银染观察细胞增殖抑制，用流式细胞术、电镜观察超微结构、免疫组织化学染色、凝集试验鉴定细胞分化。结果 SHG-44细胞经丁酸钠处理后：①细胞增殖受到明显抑制，并具有时间、剂量依赖性；②细胞周期受阻，S期细胞比例减少；③电镜观察发现细胞核变规则，异染色质增多，胞浆中线粒体、粗面内质网等细胞器增多并趋于正常；④胶质纤维酸性蛋白(Glial fibrillary acidic protein，GFAP)表达增强，改型蛋白(Vimentin)表达减弱；⑤细胞凝集度明显变低。结论 丁酸钠能抑制人脑胶质瘤SHG-44细胞的增殖，2mmol／L丁酸钠能诱导SHG-44细胞发生明显分化。

Effect of sodium butyrate on the growth and differentiation of human glioma cell line SHG-44

Objective To investigate the effect of sodium butyrate on the growth suppression and cellular differentiation of cultured SHG 44 glioma cell line Methods Human glioma SHG 44 cells were treated with 2 mmol/L sodium butyrate The inhibition of cell growth was assessed by MTT spectrophotometric analysis and AgNORs analysis The cellular differentiation was determined by flow cytometry, electron microscopy, immunohistochemical method and agglutination test Results Treatment of SHG 44 cells with sodium butyrate resulted in significant inhibition of cell growth and G1 S transition in time dependent and dose dependent manners Sodium butyrate could induce differentiation of SHG 44 cells with regularized nuclei and increased heterochromatins The mitochondria and rough endoplasmic reticulum increased in density in normal state Furthermore, SHG 44 cells expressed more GFAP but less vimentin, and the agglutination degree decreased obviously Conclusion Sodium butyrate may suppress glioma cells growth and induce differentiation of human glioma cells