色素上皮衍生因子对大鼠视网膜缺血 再灌注损伤的保护作用

目的已证实色素上皮衍生因子(pigment epithelium-deriv ed factor,PEDF)对中枢神经细胞有抗凋亡作用。本实验评价其对压力诱发的视网膜缺血再灌注的影响。方法经前房灌注维持眼压110 mm Hg (1 mm Hg = 0.133kpa)，45 min，建立大鼠视网膜缺血再灌注模型。 随后立即向玻璃体注射10　μ1(0.1μg/μl)PEDF，分别于2d和7d摘除眼球，测量塑 料包埋切片的平均视网膜内层厚度(mean thickness of inner retinal layer,MTIRL) 和视网膜节细胞(retinal ganglion cells,RGC)计数。结果PEDF玻璃体注射7d后治疗组的MTIRL和RGC明显高于对照组(118.1±5．0) μm对(949±3.0)μm, P<0.05；(6.0±1．0)个/100 μm对(4.5±0.5)个/100 μm, P<0.05]。结论玻璃体内注射PEDF有助于防止视网膜缺血再灌注后神经变性和细胞死亡。(中华眼底病杂志,2001,17:138-140)

Protective effect of pigment epithelium-derived factor on pressure-induced retinal ischemia reperfusion in rats

ObjectiveIt has been shown that pigment epithelium derived factor (PEDF) is an effective anti-apoptosis agent on several kinds of cells of the central nervous system.This study aimed to evaluate the effect of PEDF on pressure induced retinal ischemia in a rat model.MethodsRetinal ischemia was induced by increasing the intraocular pressure to 110 mm Hg for 45 minutes via an intracameral catheter.Ten microlit ers (0.1 μg/μl) PEDF was injected into the vitreous of 4 eyes of each group im mediately after reperfusion and 4 additional eyes received only normal saline as vehicle controls.The animals were euthanized at 2 or 7 days after reperfusion.T he effect of PEDF on retinal degeneration was assessed by measuring the thicknes s of the inner retinal layers (MTIRL) and counting the retinal ganglion cells (R GC) on plastic embedded retinal sections.ResultsThe MTIRL and the RGC counting in eyes treated with intravitreal PEDF were significantly higher than those in vehicle controls (118.1±5.0) μm vs(94.9±3.0) μm (P<0.05);(6.0±1.0)　cells/100　μm vs (4.5 ±0.5) cells/100　μm (P<0.05) 7 days after reperfusion,respectively．ConclusionIntravitreal administration of PEDF can ameliorate an ischemia reperfusion retinal injury and may be useful to prevent neuronal degeneration in the inner retina.(Chin J Ocul Fundus Dis, 2001,17:138-140)