MTT比色法测定膀胱癌细胞的体外侵袭力

目的　用MTT比色法与Boyden小室侵袭试验相结合，测定膀胱癌细胞的体外侵袭力。方法　采用人工基质胶构建侵袭小室，将EJ、T24、BIU-87三个膀胱癌细胞株在装有趋化物的Boyden小室内培养12h，加入MTT，用自动酶标记数仪测定吸光度，根据光密度值确定侵袭指数。结果　三个膀胱癌细胞株的侵袭指数分别为EJ0.326%±0.046%、T240.569%±0.044%、BIU-871.256%±0.066%。癌细胞趋化移动性的移动率分别为EJ0.508%±0.037%、T240.672%±0.039%、BIU-871.524%±0.043%。结论　将MTT比色法与Boyden小室侵袭试验相结合，能较准确的测定出癌细胞的体外侵袭力，具有敏感准确、快速简便、无放射污染等优点。

EVALUATION OF THE INVASIVEABILITY OF TRASITIONAL-CELL BLADDER CARCINOMA BY THE MTT COLORIMETRIC ASSAY

Objective　To evaluate the invasive ability of bladder transitional carcinoma cells(TCC),with a new method.Methods　MTT colorimetric assay combining with Boyden chamber system was used to measure the invasive ability of three bladder TCC lines EJ、T24、BIU-87. Invasive Index=experimental absorbance/control absorbance×100%.Tumor cells mortility growth curve and coloney forming test were measured for these 3 lines.Results　The Invasive Index of three cell lines were EJ 0.326%±0.046%，T24 0.569%±0.044% and BIU-871.256%±0.066%. The Motility Rate were EJ 0.508%±0.037%，T24 0.672%±0.039% and BIU-871.524%±0.043%.Conclusion　This method can evaluate the invasive ability of tumor cells accurately and it is sensitive,safe and rapid. It also can be done easily and has no radiation pollution.