| PC12细胞缺氧培养后细胞凋亡及其机制的研究 |
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| 引用本文： | 徐广润,张苏明,方思羽.PC12细胞缺氧培养后细胞凋亡及其机制的研究[J].华中医学杂志,2001,25(1):30-31. |
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| 作者姓名： | 徐广润 张苏明 方思羽 |
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| 作者单位： | 1. 山东医科大学附属医院神经内科
2. 华中科技大学同济医学院附属同济医院 |
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| 基金项目： | 国家自然科学基金!资助项目 (No .39770 810 ) |
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| 摘 要： | 目的 探讨PC12细胞缺氧后细胞凋亡及其发生机制。方法 采用末端标记法 ,结合流式细胞术观察PC12细胞缺氧培养不同时间点细胞凋亡现象 ,以及DNA损伤相关基因表达的改变。结果 在缺氧 0 .5h开始出现凋亡细胞 ,p5 3、p2 1wafl/cipl蛋白表达也开始增高。至缺氧 1h凋亡细胞达高峰 ,p5 3、p2 1蛋白表达亦最高 (P <0 .0 1) ,缺氧 6～ 12h ,则以坏死为主 ,以上基因的表达均减弱。结论 缺氧后PC12细胞凋亡部分是由于DNA损伤严重、损伤不能及时得到修复所致。
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| 关 键 词： | 缺氧 细胞凋亡 DNA损伤 细胞培养 PC12细胞 |
| 修稿时间： | 2000年8月20日 |
The study on the PC12 cell apoptosis and its mechanism after hypoxic culture |
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| Xu Guangrun,Zhang Suming,Zhang Min et al.The study on the PC12 cell apoptosis and its mechanism after hypoxic culture[J].Central China Medical Journal,2001,25(1):30-31. |
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| Authors: | Xu Guangrun Zhang Suming Zhang Min |
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| Institution: | Xu Guangrun,Zhang Suming,Zhang Min et al. Department of Neurology,Tongji Medical College of Huazhong University of Science and Technology,Wuhan 430030 |
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| Abstract: | Objective To investigate the PC12 cell apoptosis and its mechanism after hypoxic culture.Methods The apoptosis of PC12 cells in different time points after hypoxic culture was observed by TUNEL techniques and the expression of DNA damage related genes detected by flow cytometry techniques.Results Apoptotic cells appeared at 30 min after hypoxic culture and the expression of protein p53, p21 began to be increased. Apoptosis cells reached the peak and the expression of proteins p53, p21 walf/cipl arrived the top at 1 h following hypoxia ( P < 0.01 ). At 6 h and 12 h after hypoxia, PC12 cells were mainly necrostic and expression of the genes was also decreased.Conclusion PC12 apoptosis following hypoxia was partly contributed to serious DNA damage which could not be repaired immediately. |
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| Keywords: | Hypoxia Cell apoptosis DNA damage Cell culture |
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