胃动素对大鼠海马神经元放电活动的影响

目的研究大鼠海马胃扩张神经元的特点及胃动素对胃扩张神经元放电活动的影响。方法采用微电极细胞外记录方法在体观察胃动素是否对82只大鼠海马胃扩张(GD)神经元放电有影响,胃扩张神经元根据刺激后的变化可分为胃扩张兴奋性神经元和胃扩张抑制性神经元。结果①在82只大鼠上记录到的358个海马单位电活动,根据神经元放电模式可分为位相性、连续性和单个性。神经元放电频率可分为:高频(>10Hz)、中频(5～10Hz)和低频(0～5Hz)。②海马内358个对GD刺激有反应的171个GD神经元中,放电频率增加的海马神经元有130个(70.5±4.8)%,为GD兴奋性神经元(GD-E);GD引起海马神经元放电频率减少的神经元41个(-60.3±4.2)%,为GD抑制性神经元(GD-I);③海马CA1区内的66个GD-E神经元中有38个(57.6%)对胃动素呈现兴奋性效应;在18个GD-I神经元中有10个神经元(55.6%)表现为兴奋效应,6个神经元放电频率降低。结论海马区有胃扩张反应性神经元;胃动素对海马胃扩张神经元有兴奋作用。

Effect of Motilin on Electric Motility of Hippocampal Neurons in Rats

Objective To investigate the characteristics of hippocampus neurons charging of gastrectasis and its firing activity influenced by motilin. Methods Electrophysiological experiment：In 82 rats,extra cellular recordings in vivo were made from hippocampus using microelectrode. Neurons were categorized as gastile distension excitatory. （GD-E） or inhibitory （GD-I） neurons tested with gastric distension stimulus. Results ① In 82 rats, spontaneous discharge was recorded from 358 neurons. The patterns of electric activity of neurons showed in phasic, continuous and single firing 3 types. The frequency of cell firings have been classified as high（ 〉 10 Hz） ,intermediate（5 - 10 Hz） and low（0 -5 Hz） frequency group; ②Motilin can excited the discharge activity of gastric distension neurons. There were 66 neurons in hippocampal responded to the gastric distension stimulation （GD）. 38 of them （38/66,57.6%） showed excitatory response to GD classfied as GD-E neuren. And 10 of neurons （10/18,55.6%） excited by GD stimulation as GD-I neurons. Conclusion There are GD neurons in hippocampus; motilin can stimulate the spontaneous discharge of gastric dilatation neurons in hippocampus.