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| pcDNA3.1-BMP-2真核表达载体构建及转染兔骨髓基质细胞的实验研究 | |
| 引用本文： | 付春江 毕郑钢 张军 杨成林 曹杨 田方 陶天遵. pcDNA3.1-BMP-2真核表达载体构建及转染兔骨髓基质细胞的实验研究[J]. 中国骨质疏松杂志, 2004, 10(1): 39-41,51 |
| 作者姓名： | 付春江 毕郑钢 张军 杨成林 曹杨 田方 陶天遵 |
| 作者单位： | 1. 150001,哈尔滨医科大学第一临床医学院骨科 2. 150001,哈尔滨医科大学,军事医学科学院307医院 3. 150001,哈尔滨医科大学,第二临床医学院骨科 |
| 摘 要： | 目的 构建人骨形态发生蛋白2(BMP-2)真核表达载体，鉴定并转染兔骨髓基质细胞，为转基因治疗骨缺失疾病提供实验基础。方法 双酶切克隆载体将BMP-2目的基因与真核表达载体pcDNA3．1连接，免疫组化及Western blotting检测转染BMP-2基因骨髓基质细胞的表达效果。结果 成功构建BMP-2真核表达载体，转染骨髓基质细胞后，有BMP-2表达。结论 骨髓基质细胞经基因转染后可以表达BMP-2。为进一步实验研究提供基础。 |
| 关 键 词： | pcDNA3.1-BMP-2 真核表达载体 基因转染 兔 骨髓基质细胞 实验 骨形态发生蛋白2 骨质疏松 |
| 修稿时间： | 2003-08-13 |
Construction of mammal expression plasmid pcDNA3.1-BMP-2 and transection of bone marrow stromal cells |
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| FU Chunjiang,BI Zhenggang,ZHANG Jun,et al.. Construction of mammal expression plasmid pcDNA3.1-BMP-2 and transection of bone marrow stromal cells[J]. Chinese Journal of Osteoporosis, 2004, 10(1): 39-41,51 | |
| Authors: | FU Chunjiang BI Zhenggang ZHANG Jun et al. |
| Affiliation: | FU Chunjiang,BI Zhenggang,ZHANG Jun,et al. Department of Orthopedics,The First Clinical College,Harbin Medical University,Harbin 150001,China |
| Abstract: | Objective To construct mammal expression plasmid vector containing human BMP-2 for the study of gene therapy of bone defect disease. Methods Clone plasmid DNA pspBMP-2 was digested by Xba I and Sal I, recombined with plasmid pcDNA3.1, and the plasmid was transfected into rabbit bone marrow stromal cells. The expression of bone morphogenetic protein-2 was detected by immunochemistry and Western blotting. Results After BMP-2 expression plasmid was constracted, bone morphogenetic protein-2 was detected in bone marrow stromal cells. Conclusion Bone marrow stromal cells can express bone morphogenetic protein-2 after transfec-tion. This method provides a basis for studying BMP-2. |
| Keywords: | Bone morphogenetic protein-2 Stroma Transfection |
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