补肾活血泄浊汤对体外培养肾小球上皮细胞增殖影响

目的:探讨脂多糖(LPS)、硫酸鱼精蛋白、表皮生长因子(EGF)及肿瘤坏死因子-α(TNF-α)及补肾活血泄浊汤对体外培养大鼠肾小球上皮细胞生长的影响。方法:应用LPS、硫酸鱼精蛋白、EGF及TNF-α作为刺激因子,观察其对肾小球上皮细胞(GEC)增殖的影响;并采用血清药理学方法,提取动物的含药血清作用于上述受刺激的上皮细胞,观察补肾活血泄浊汤对GEC增殖的影响。结果:LPS、EGF+TNF-α和硫酸鱼精蛋白均可抑制GEC掺入³H]-TdR,并呈一定的量效和时效关系;而补肾活血泄浊汤可使GEC掺入³H]-TdR增加。结论:GEC是补肾活血泄浊汤发挥治疗作用的重要靶细胞,这可能是该方防治以蛋白尿为主要临床表现的肾小球疾病的机制之一。

Effect of "Bushen Huoxue Xiezhuo" decoction on growth of rat glomerular epithelial cells

AIM:To examine the effect of protamine sulfate, lipopolysaccharide(LPS),tumor necrosis factor(TNF), epidermal growth factor(EGF) and "Bushen Huoxue Xiezhuo"(BHX) decoction on the proliferation of extracorporeal cultured rat glomerular epithelial cells (GEC). METHODS:Their action on the proliferation of rat GEC were investigated using the ³H]-TdR incorporation. Meanwhile, the serum of rats treated with BHX decoction was extracted pharmacologically and its effects on the growth of GEC were also studied. RESULTS:LPS, protamine sulfate, TNF-α and EGF could significantly inhibit the ³H]-TdR incorporation of GEC in a dose- and time-dependent manner. However, this inhibition could be efficiently reversed by the serum containing BHX decoction. CONCLUSION:GEC is one of the main target cells on which BHX decoction act, and the protection on GEC might be one of the mechanisms underlying the role of BHX decoction in preventing the progression of nephrosis.