糖脂清颗粒对 ２型糖尿病大鼠心肌组织 Bax、Bcl ２表达的影响

目的：观察糖脂清颗粒对２型糖尿病大鼠心肌组织 Bax、Bcl ２表达的影响，并探讨其干预心肌细胞凋亡的机制。方法：雄性ＳＤ大鼠，采用高糖高脂饲料喂养８周后，一次性小剂量（３０ｍｇ·ｋｇ^－１）２％ 链脲佐菌素（ＳＴＺ）溶液腹腔注射造模，３ｄ后尾尖采血，测随机血糖≥16.7ｍｍｏｌ·Ｌ^－１者确定为２型糖尿病大鼠模型，８周后，用免疫组织化学法检测各组心肌组织 Bcl-２、Bax的表达。结果：糖脂清颗粒各治疗组大鼠心肌组织 Bcl-２表达明显上升，与模型组比较，差异有统计学意义（Ｐ＜0.05）；各治疗组大鼠心肌组织 Bax／Bal-２明显下降，与模型组比较，差异有统计学意义（Ｐ＜0.05）。结论：糖脂清颗粒具有抗糖尿病大鼠心肌细胞凋亡的作用，其可能机制为升高 Bcl-２的表达，从而降低 Bax／Bcl-２的比值，抑制心肌细胞凋亡，延缓糖尿病心肌病的进展。

Influence of Tangzhiqing Granule on the Expression of Bax and Bcl-2 in Myocardium of Type 2 Diabetic Rats

Objective ： To observe the effect of Tangzhiqing Granule on the expression of Bax and Bcl-2 in myocardium of type 2 diabetic rats, and to explore the intervention of myocardial cell apoptosis mechanism. Methods ： Male SD rats with high fat and high sugar diet for 8 weeks, disposable small dose （ 30 mg·kg^-1 ） 2% streptozotocin （STZ） solution intraperitoneal injection for molding, after 3 days, collection the tail tip blood, measuring random blood glucose ≥16.7 mmol·L^-1 to identify type 2 diabetes rat model, and 8 weeks later, the expression of immunohistoehemistry was used to detect Bcl-2 and Bax in myocardial tissue in each group. Results ： The expressions of BcL-2 in myocardium of rats in Tangzhiqing Granule each treatment group were increased significantly, compared with the model group, the difference was statistically significant（ P 〈 0.05 ） ;and Bax/Bcl-2 were decreased significantly, compared with the model group, the difference was statistically significant（ P 〈 0.05 ）. Conclusion：Tangzbiqing Granule has the effect of anti diabetic rats myocardial cell apoptosis, and the possible mechanism is increasing the expression of Be1-2, thereby reducing the ratio of Bax/Bcl-2, inhibiting the apoptosis of myocardial cells, and delaying the progression of diabetic cardiomyopathy.