| p53在蛛网膜下腔出血小鼠脑皮质的表达及其与PI3K/Akt信号通路的关系 |
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| 引用本文： | 李晓晖,李冉,田艳霞,崔颖,崔建忠,王凯杰,高俊玲.p53在蛛网膜下腔出血小鼠脑皮质的表达及其与PI3K/Akt信号通路的关系[J].解剖学报,2011,42(4):446-450. |
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| 作者姓名： | 李晓晖 李冉 田艳霞 崔颖 崔建忠 王凯杰 高俊玲 |
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| 作者单位： | 1.河北联合大学基础医学部，河北 唐山 063000; 2.河北医科大学临床医学系, 石家庄 050017; 3.唐山市工人医院神经外科，河北 唐山 063000; 4.河北省煤矿卫生与安全实验室，河北 唐山 063000 |
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| 基金项目： | 河北省自然科学基金资助项目，河北省教育厅重点课题资助项目，卫生部人类疾病比较医学重点实验室开放课题资助项目 |
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| 摘 要： | 目的 探讨p53蛋白在实验性蛛网膜下腔出血小鼠脑皮质的表达及其与PI3K/Akt信号通路的关系.方法 将72只小鼠随机平均分为3组,即假手术组(Sham组),蛛网膜下腔出血组(SAH组),抑制剂LY294002组(LY组).采用线栓法制备小鼠蛛网膜下腔出血模型,术后观测6个时相点:0h、3h、6h、9h、12h、24h...
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| 关 键 词： | 蛛网膜下腔出血 p53 Akt 免疫组织化学 免疫印迹法 小鼠 |
| 收稿时间： | 2011-01-20 |
Expression of p53 and its relationship with PI3K/Akt signal pathway in subarachnoid hemorrhage in mice cortex |
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| LI Xiao-hui,LI Ran,TIAN Yan-xia,CUI Ying,CUI Jian-zhong,WANG Kai-jie,GAO Jun-ling.Expression of p53 and its relationship with PI3K/Akt signal pathway in subarachnoid hemorrhage in mice cortex[J].Acta Anatomica Sinica,2011,42(4):446-450. |
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| Authors: | LI Xiao-hui LI Ran TIAN Yan-xia CUI Ying CUI Jian-zhong WANG Kai-jie GAO Jun-ling |
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| Institution: | 1.Department of Basic Medicine, Hebei United University, Hebei Tangshan 063000, China;2.Department of Clinical Medicine, Hebei Medical University, Shijiazhuang 050017, China;3.Department of Neurosurgery, Tangshan Gongren Hospital, Hebei Tangshan 063000, China;4.Hebei Province Coal Mine Health and Safety Laboratory, Hebei Tangshan 063000, China |
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| Abstract: | Objective To explore the expression of p53 protein and its relationship with the PI3K/Akt signal pathway in subarachnoid hemorrhage (SAH). Methods SAH was induced in mice by puncture of internal carotid artety. Seventy-two male mice were randomly divided into sham group, SAH group, LY (SAH+LY294002) group. The animals were sacrified at 0, the 3rd, the 6th, the 9th, the 12th and the 24th hour, to observe the expression of p53 and p-Akt in the brain with immunohistochemisty and Western blotting. The morphological changes of the neurons were studied by light microscope. RT-PCR was preformed for detecting the expression change of p53 and p-Akt mRNA. Results Neurons in the hippocampal CA1 region after SAH showed typical apoptotic features under light microscope. The expression of p53 and p-Akt protein was higher in SAH group than in sham group. Compared with SAH group, the expression of p-Akt decreased while the p53 was increased with the extension of time in LY group. Conclusion p53 and p-Akt are increased in cerebral cortex of mice after SAH. PI3K/Akt signal pathway could protect the brain neurons by modulating the p53 expression, which could induce the cell apoptosis. |
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| Keywords: | p53 Akt |
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