| 腹股沟区皮下埋藏睾丸对生精细胞凋亡及Bcl-2/Bax蛋白表达的影响 |
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| 作者姓名： | Cui HW Wang DL Sun GF Wei ZR |
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| 作者单位： | 1. 遵义医学院附属医院整形外科,贵州遵义563003;新乡医学院第一附属医院手外科,河南新乡453100
2. 遵义医学院附属医院整形外科,贵州遵义,563003 |
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| 基金项目： | 贵州省优秀科技教育人才省长专项基金(2005-65) |
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| 摘 要： | 目的:通过观察腹股沟区皮下埋藏睾丸生精细胞的凋亡及Bcl-2/Bax蛋白表达,探讨二者的关系。方法:以健康育龄期36只雄性新西兰大白兔为实验动物,随机分成实验组18只、对照组18只。实验组动物将双侧睾丸分别移位埋藏至双侧腹股沟区皮下,以制备睾丸埋藏修复阴囊皮肤缺损模型;对照组未作处理。动物模型建立后第8周末,每组随机取6只大白兔测量睾丸表面温度后进行睾丸活检。睾丸组织行原位末端标记(TUNEL)法检测生精细胞凋亡、免疫组化法结合图像分析仪检测睾丸生精细胞Bcl-2、Bax蛋白的表达。结果:对照组的睾丸表面温度为(36.15±0.64)℃,实验组为(38.02±0.36)℃,两组比较差异有统计学意义(P<0.05)。模型建立后第8周末实验组睾丸生精小管中生精细胞的凋亡指数(AI)为(89.69士3.76)%,对照组为(7.73±4.95)%,两者比较有显著差异(P<0.05)。实验组Bax表达显著升高(P<0.05),Bcl-2表达显著降低(P<0.05),凋亡细胞主要为初级精母细胞和圆形精子细胞。结论:腹股沟区皮下埋藏睾丸局部温度升高诱导睾丸的生精细胞凋亡增加,生精细胞凋亡增加与Bcl-2蛋白表达降低、Bax蛋白表达升高密切相关。Bcl-2/Bax的改变是高温引起生精细胞凋亡的机制之一。
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| 关 键 词： | 睾丸 埋藏 生精细胞 凋亡 Bcl-2 Bax 新西兰大白兔 |
Spermatogenic cell apoptosis and expressions of Bcl-2 and Bax proteins after burying the testis in the inguinal pocket |
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| Cui HW,Wang DL,Sun GF,Wei ZR.Spermatogenic cell apoptosis and expressions of Bcl-2 and Bax proteins after burying the testis in the inguinal pocket[J].National Journal of Andrology,2011,17(9):785-789. |
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| Authors: | Cui Hong-Wang Wang Da-Li Sun Guang-Feng Wei Zai-Rong |
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| Institution: | CUI Hong-wang1,2,WANG Da-li1,SUN Guang-feng1,WEI Zai-rong11.Department of Plastic Surgery,Affiliated Hospital of Zunyi Medical College,Zunyi,Guizhou 563003,China,2.Department of Hand Surgery,The First Affiliated Hospital of Xinxiang Medical University,Xinxiang,Henan 453100 |
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| Abstract: | Objective: To observe the apoptosis of spermatogenic cells and the expressions of Bcl-2 and Bax proteins after burying the testis in the inguinal pocket,and to investigate their relationship.Methods: We randomly divided 36 healthy male New Zealand white rabbits into an experimental group(n=18) and a control group(n=18).Models were established by burying testes in the inguinal pocket in the experimental group,while the controls were left untreated.At the end of the 8th week after surgery,6 animals were rando... |
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| Keywords: | testis burying spermatogenic cell apoptosis Bcl-2 Bax New Zealand white rabbit |
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