槲皮素对前列腺癌PC-3细胞凋亡作用的研究

目的:研究槲皮素对人前列腺癌PC-3细胞的凋亡作用。方法:体外培养PC-3细胞,给予不同浓度(50、100、150、200、250μmol/L)的槲皮素处理,MTT法检测槲皮素对细胞抑制率;流式细胞仪检测细胞凋亡率;透射电镜观察细胞超微结构的变化。结果:槲皮素能抑制人前列腺癌PC-3细胞的体外生长,且呈时间与剂量依赖性,浓度由低到高,其24 h的抑制率(%)分别为3.01±1.32、4.84±1.73、20.35±1.30、16.78±1.89、27.25±4.01,48 h的抑制率(%)分别为10.18±1.16、6.22±0.04、24.29±4.19、22.4±4.26、41.42±5.43,当药物浓度>150μmol/L时P<0.05,具有统计学意义;流式细胞术显示PC-3细胞凋亡率随槲皮素浓度升高和作用时间延长而增加(P<0.05),其中浓度150μmol/L和200μmol/L组,24 h的凋亡率(%)分别19.10±0.28、26.55±0.78,48 h的凋亡率(%)分别为27.65±1.06、38.30±5.96;电镜观察到细胞的典型凋亡形态学变化。结论:在适当的条件下,槲皮素对人前列腺癌PC-3细胞增殖有明显的抑制作用,同时可诱导PC-3细胞凋亡,其具体作用机制有待进一步深入研究。

Quercetin induces the apoptosis of human PC-3 cells

Objective： To study the effect of quercetin on the apoptosis of human PC-3 cells.Methods： Human PC-3 cells were cultured in vitro and then treated with quercetin at the concentrations of 50,100,150,200 and 250 μmol/L.The inhibition rate of quercetin on the PC-3 cells was detected by MTT,the apoptosis of the cells determined by flow cytometry,and the changes of the cellular ultramicrostructure observed by transmission electron microscopy.Results： Quercetin markedly inhibited the proliferation of PC-3 cells in vitro in a time-and dose-dependent manner.Its inhibition rates were（3.01±1.32）%,（4.84±1.73）%,（20.35±1.30）%,（16.78±1.89）% and（27.25±4.01）% at 24 hours,and（10.18±1.16）%,（6.22±0.04）%,（24.29±4.19）%,（22.4±4.26）% and（41.42±5.43）% at 48 hours in the 50,100,150,200 and 250 μmol/L groups,respectively,with statistical significance at the concentration of 150 μmol/L（P0.05）.Flow cytometry showed that the apoptosis of PC-3 cells was increased with the elevated concentration and prolonged time of Quercetin treatment,（19.10±0.28）% and（26.55±0.78）% at 24 hours,and（27.65±1.06）% and（38.30±5.96）% at 48 hours in the 150 and 200 μmol/L groups,respectively（P0.05）.Typical changes in the morphology of the cells were observed under the transmission electron microscope.Conclusion： Quercetin can inhibit the proliferation and induce the apoptosis of human PC-3 cells,but its action mechanism remains to be further investigated.