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| 结核杆菌耐药性调查与基因突变分析 | |
| 引用本文： | 刘灵辉,谷素英,徐亚军,刘衡川.结核杆菌耐药性调查与基因突变分析[J].检验医学与临床,2010,7(7):593-595. |
| 作者姓名： | 刘灵辉 谷素英 徐亚军 刘衡川 |
| 作者单位： | 1. 广东省深圳市宝安区福永预防保健所,518103 2. 广东省深圳市龙岗区疾病预防控制中心,518172 3. 四川大学华西公共卫生学院,成都,610041 |
| 摘 要： | 目的了解结核分支杆菌耐药性与基因突变情况,分析耐药表型与基因突变的相关性。方法从临床标本中分离培养、鉴定结核分支杆菌;应用药敏试验、单链探针反向杂交试验技术(LiPA)和基因测序分析结核分支杆菌耐药性与基因突变情况。结果50株结核分支杆菌中,12株耐异烟肼(INH),7株耐利福平(RFP),15株耐链霉素(SM),2株耐乙胺丁醇(EMB)。LiPA检测耐RFP菌株,4株rpoB基因531位TCG→TTG突变;耐INH菌株中5株KatG基因315位AGC→ACC突变;耐SM菌株中6株、SM敏感株1株rpsl基因43位AAG→AGG突变,1株耐SM菌株rpsl基因88位AAG→AGG突变;rrs基因未检测到突变;1株耐EMB菌株与1株EMB敏感株的embB基因306位发生ATG→GTG突变。结论本市结核分支杆菌的耐药性增加的趋势显著,加强监测与综合干预非常必要。 |
| 关 键 词： | 结核杆菌 耐药性 基因突变 单链探针反向杂交试验技术 |
Analysis on the drug resistance and drug-resistant gene mutations in mycobacterium tuberculosis |
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| LIU Ling-hui,GU Su-ying,XU Ya-jun,LIU Heng-chuan.Analysis on the drug resistance and drug-resistant gene mutations in mycobacterium tuberculosis[J].Laboratory Medicine and Clinic,2010,7(7):593-595. | |
| Authors: | LIU Ling-hui GU Su-ying XU Ya-jun LIU Heng-chuan |
| Institution: | LIU Ling-hui1,GU Su-ying1,XU Ya-jun2,LIU Heng-chuan3.1.Center of Disease Control , Prevention in Fuyong,Shenzhen,518103,China,2.Center of Disease Control , Prevention in Longgang Shenzhen 518172,3.Department of Medical Technology of the Public Health School of Sichuan University,Chengdu Sichuan 610041 |
| Abstract: | Objective To investigate the mutations situation of streptomycin-resistant genes in M.tuberculosis and analyse the correlation of the results of traditional drug-susceptibility test.Methods The traditional cultivation,drug-susceptibility test and LiPA were performed in 50 M.tuberculosis.Results The traditional drug-susceptibility showed that there were 7 strains of rifampicin resistants,12 strains of isoniazid resistants,15 strains of streptomycin resistant strains and 2 ethambutol resistant strains.50 M.tu... |
| Keywords: | mycobacterium tuberculosis streptomycin drug-resistance PCR-LiPA |
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