Urinary free light chain analysis by the Freelite immunoassay: a preliminary study in multiple myeloma |
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| Authors: | Le Bricon Thierry Bengoufa Djaouida Benlakehal Mourad Bousquet Bernard Erlich Danielle |
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| Affiliation: | Laboratoire de Biochimie A, H?pital St-Louis AP-HP, 1 avenue Claude Vellefaux, 75475 Paris Cedex 10, France. thierry.le-bricon@sls.ap-hop-paris.fr |
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| Abstract: | Evaluate the Freelite free light chain immunoassay for urine analysis in myeloma.Urine specimens from 20 patients were analyzed by Freelite (The Binding Site) and SDS-agarose gel electrophoresis (Hydragel protéinurie, Sebia).Using the kappa/lambda ratio, Freelite was more sensitive than electrophoresis to detect free light chains, but concentration was overestimated in 75% of cases.Despite high sensitivity and full automation, Freelite inaccurately measures monoclonal free light chains in urine. |
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| Keywords: | Free light chain Electrophoresis Urine Multiple myeloma |
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