基于体外心肌细胞活力的葛根提取工艺优选

目的:优选葛根药材的提取工艺.方法:采用正交试验法,选取提取时间、料液比、提取次数及乙醇体积分数为影响因素,以葛根素含量和药效学的体外心肌细胞活力为指标综合分析,优选提取工艺条件.结果:葛根的乙醇提取最佳工艺条件为加6倍量70％乙醇回流提取2次,每次1.5h.结论:化学指标结合药效学指标综合分析葛根的提取纯化工艺可较好地保证制剂的质量,且该优选工艺稳定可行.

Optimization of Extraction Process for Pueraria lobata Based on Viability of in vitro Myocardial Cell

Objective: To optimize extraction technology of Pueraria lobata. Method: With the content of puerarin and pharmacodynamics of in vitro myocardial cell viability as comprehensive analysis indexes,orthogonal test was used to optimize extraction technology with extraction time,solid-liquid ratio,extraction times and the concentration of ethanol as factors. Result: The best extract condition of P. lobata was:refluxing extracted 2 times with 6 times the amount of 70% ethanol,1.5 h per time. Conclusion: Combining with chemical and pharmacodynamic indexes to optimize extraction and purification process could better guarantee quality of preparations,and this optimized technology was stable and feasible.