四逆散传统饮片汤剂和配方颗粒对两种因素致小鼠肝损伤模型的药效比较

目的:四逆散传统汤剂和配方颗粒是在临床中同时存在的两种用药形式,本研究拟对比研究四逆散传统汤剂和配方颗粒的疏肝解郁药效作用,为四逆散配方颗粒的临床使用提供实验依据.方法:昆明种小鼠随机分为空白组、应激模型组、阳性药组、四逆散传统汤剂低、高剂量(600,1200 mg·kg-1)组、四逆散配方颗粒汤剂低、高剂量(600,1200 mg·kg-1)组共7组,每组10只.小鼠连续灌胃给药1周,实验第6天时给予小鼠一次性拘束应激18h,测定血浆丙氨酸转氨酶(ALT)和天冬氨酸转氨酶(AST)水平.昆明种小鼠随机分为空白组、粉刺杆菌-脂多糖(Propionibacterium acne-LPS)致肝炎模型组、阳性药组、四逆散传统汤剂低、高剂量(600,1200 mg· kg-1)组、四逆散配方颗粒汤剂低、高剂量(600,1200 mg· kg-1)组共7组,每组10只.小鼠尾静脉注射20 mg·kg-1热灭活粉刺杆菌生理盐水溶液5d后,尾静脉注射5μg· kg-1脂多糖生理盐水溶液,5h测定血浆ALT,AST水平.结果:与正常对照组相比,应激模型组和P.acnes-LPS的血浆ALT均显著增高(43.4±2.0)U·L-1vs (12.2-2.8) U·L-1,P ＜0.05；(114.9±23.3) U·L-1vs (18.8±5.2) U·L-1,P ＜0.05,AST水平也显著增高.与模型组相比,四逆散传统饮片汤剂和配方颗粒均能显著降低应激负荷和粉刺杆菌-脂多糖升高的血浆ALT和AST水平,但是两药药效没有统计学差异.结论:四逆散传统饮片汤剂和配方颗粒均有较好的保肝作用.

Comparison of the Liver-protective Effects between Traditional Decoction and Chinese Medicinal Dispensing Granule of Sini San

Objective:To compare the liver-protective effects between traditional decoction and Chinese medicinal dispensing granule of Sini San.Method:Liver injury models were induced by establishing restraint stress and Propionibacterium acnes-lipopolysaccharide(LPS) in mice.Kunming mice were divided into 7 groups,normal control,stress model,positive control,Sini San decoction(600,1 200 mg · kg-1) and Sini San dispensing granule(600,1 200 mg · kg-1).Mice were administered for consecutive 6 days and restrained for 18 h.Hepatic function was evaluated by assessing assessing alanine aminotransferase(ALT) and aspartate aminotransferase(AST) level in plasma.Kuming mice were divided into 7 groups,such as normal control,P.acnes-LPS model,positive control,Sini San decoction(600,1 200 mg · kg-1) and Sini San dispensing granule(600,1 200 mg · kg-1).Mice were injected with 20 mg · kg-1 P.acnes and 5 μg · kg-1 LPS to induce liver injury.Hepatic function was evaluated by ALT and AST level in plasma.Result:Compared with the model groups,the traditional decoction and Chinese dispensing granule of Sini San significantly reduced ALT and AST levels induced by restraint stress or P.acne-LPS.However,there were no significant difference between the two samples.Conclusion:The traditional decoction and Chinese medicinal dispensing granule of Sini San both are effective on improving the liver function in liver-injury mice model.