麻杏石甘汤对LPS诱导的大鼠发热模型的影响

目的:建立静脉注射内毒素的大鼠发热模型,观察麻杏石甘汤的解热作用.方法:大鼠尾静脉注射多个浓度(5,10,20,40,60,80μg·kg-1)的内毒素,观察各组大鼠的体温变化,建立适宜的尾静脉注射脂多糖(LPS)发热模型；将大鼠随机分为空白组,模型组,给药组(低、中、高剂量分别为1.33,4.5,15.2 g·kg-1),实验当天灌胃给药后立即造模,观察麻杏石甘汤对该发热模型动物体温变化的影响.结果:静脉注射内毒素可引起SD大鼠显著升温(P＜0.01),结果表明静脉注射内毒素致SD大鼠发热模型最佳造模剂量为20 μg·kg-1.灌服低、中、高剂量麻杏石甘汤的大鼠的体温均较模型组明显降低(P＜0.05).比较各组的体温反应指数TRI值,发现各组的解热效应有明显的剂量效应关系.结论:麻杏石甘汤对静脉注射LPS诱导的大鼠发热模型具有较好的解热作用,且呈剂量依赖性.

Antifebrile Effect of Maxing Shigan Tang on Fever Induced by LPS in Rats

Objective:Using rat intravenous endotoxin model of fever to observe the antifebrile effect of Maxing Shigan Tang.Method: the effects of Maxing Shigan Tang on body temperature was observed on the fever induced by intravenous injection of lipopolysaccharide(LPS) at the dose 5,10,20,40,60,80 μg · kg-1,respectively.The rats were randomly divided into blank group,model group,treatment group(low,medium and high dose respectively 1.33,4.5,15.2 g · kg-1),the model was induced immediately after administration.Result: Intravenous injection of endotoxin could increase body temperature significantly(P<0.01).The results showed that the dose of intravenous injection of endotoxin induced SD rat fever model was 20 μg · kg-1.The body temperature was significantly lower compared with model group after fed with low,medium and high dose of Maxing Shigan Tang(P<0.05).TRI showed a clear dose-response relationship.Conclusion: Maxing Shigan Tang has a better antifebrile effects on the fever induced by intravenous injection of LPS in rats,and show a dose-dependent manner.