芎归舒络颗粒提取纯化工艺考察

目的:优选芎归舒络颗粒的提取、纯化工艺.方法:以阿魏酸转移率为指标,采用正交试验优选芎归舒络颗粒水提取工艺,选取浸泡时间、加水量、提取次数及提取时间为影响因素,HPLC测定阿魏酸含量；采用醇沉法纯化芎归舒络颗粒,以阿魏酸转移率和纯度为指标,单因素试验考察其醇沉条件.结果:优选的提取工艺为加15倍量水浸泡1h,提取2次,每次1h;其醇沉条件为醇沉至乙醇体积分数60％,相对密度1.08 ～ 1.14(60℃).结论:该优选工艺稳定可行,可为芎归舒络颗粒的工业化生产提供试验依据.

Investigation of Extraction and Purification Technology for Qionggui Shuluo Granule

Objective:To optimize extraction and purification technology of Qionggui Shuloug granule.Method:With transfer rate of ferulic acid as index,water extraction technology of Qionggui Shuloug granule was optimized by orthogonal test,soaking time,extraction times,extraction time and the amount of water were selected as factors,the content of ferulic acid was determined by HPLC;With transfer rate and purity of ferulic acid as indexes,single-factor test was used to investigate alcohol precipitation technology.Result:Optimized extraction technology was:soaked 1 h with 15 times the amount of water,extracted 2 times with 1 h per time;alcohol precipitation technology was:alcohol precipitation to the concentration of ethanol 60%,the relative density of 1.08-1.14(60 ℃).Conclusion:This optimized technology was stable and feasible,it could provide experimental basis for industrial production of Qionggui Shuloug granule.