二紫胶囊对生精障碍大鼠凋亡调控基因的影响

目的:观察二紫胶囊对生精障碍大鼠凋亡调控基因的影响.方法:将40只健康雄性大鼠分为模型组、对照组、二紫胶囊高、低剂量组,以环磷酰胺注射液40 mg· kg-1,ip制造大鼠生精障碍模型,分别ig生理盐水、二紫胶囊浸膏(4.8,2.4g·kg-1),连续60 d,用TUNEL法检测大鼠睾丸生精细胞凋亡,免疫组化法测定Bax和Bcl-2蛋白在大鼠睾丸组织中的表达.结果:与模型组相比较,二紫胶囊高、低剂量组生精细胞凋亡指数明显下调(7.16±1.47)％,(13.83±2.40)％ vs(20.50±2.17)％],Bax蛋白表达明显下调(16.01±2.02),(26.67±1.79)vs(9.94±1.14)](P＜0.01),Bcl-2蛋白表达明显上调(40 12±3.06),(22.87±2.94)vs(16.26±1.03)](P＜0.01).结论:二紫胶囊能通过下调Bax和上调Bcl-2表达,减少生精细胞凋亡的发生.

Effect of Erzi Capsule on Apoptosis Genes in Rat with Spermatogenic Failure

Objective: To investigate effects of Erzi Capsule (EZC) on apoptosis genes in rats with spermatogenic failure and to explore the related mechanism of action for treatment of male infertility. Method: Forty male rats were randomly divided in to 4 groups:model group, control group and two EZC dose groups (4.8,2.4 g · kg^-1). The rat model of spermatogenic failure was established by intraperitoneal injection of cyclophosphamide (40 mg · kg^-1). The treatments were lasted for 60 days. TUNEL method was used to determine apoptosis in spermatogenic cells. Bax and Bcl-2 expressions in testis were tested by immunohistochemical method. Result: Compared with the model group, two doses of EZC group decreased apoptosis index, lowered Bax expression and increased Bcl-2 expression significantly(P<0.01). Conclusion: EZC could downregulate the expression of Bax and upregulation Bcl-2 expression, reduce the incidence of apoptosis in spermatogenic cells.