阳荷提取物体外抗菌作用研究

目的:研究姜科姜属植物阳荷根茎乙醇提取物的体外抗菌活性.方法:运用乙醇渗漉法提取得到阳荷总提取物,依次用石油醚、氯仿、乙酸乙酯、正丁醇萃取,得到不同极性部位；用滤纸片法对乙醇提取物及各萃取物进行体外抗菌活性测定,有抑菌作用的萃取物进一步用微量肉汤稀释法测定其最低抑菌浓度(MIC)和最低杀菌浓度(MBC).结果:阳荷根茎乙醇提取物、石油醚和氯仿萃取物具有抑制金黄色葡萄球菌等3种革兰阳性菌和大肠埃希菌等3种革兰阴性菌生长繁殖的活性,MIC值在312.5 ～1250 mg·L-1.总提取物对地衣芽孢杆菌、无乳链球菌和大肠埃希菌的MBC均为1250 mg· L-；石油醚和氯仿萃取物对6种测试菌株均有杀灭作用,MBC值在625～1250 mg·L-1.结论:阳荷根茎乙醇提取物及石油醚和氯仿萃取物能显著抑制金黄色葡萄球菌、地衣芽孢杆菌、无乳链球菌、大肠埃希菌、肺炎克雷伯菌和铜绿假单胞菌的生长繁殖,具有明显的灭活作用.

Experimental Study on in vitro Antibacterial Effect of Extract from Rhizoma Zingiber striolatum

Objective: To study in vitro bacteriostasis of ethanol extract from the rhizomes of Zingiber striolatum Diels. Method: The rhizomes were percolated with ethanol and then the residue was sequentially extracted with petroleum ether, chloroform, ethyl acetate and n-butanol after reduce-pressure distillation. The ethanol extract and various polar fractions were screened for in vitro antibacterial activities by disc-diffusion method. The minimum inhibition concentrations (MICs) and minimum bacteriocidal concentrations (MBCs) were further determined for active polar fractions by broth microdilution method. Result: The crude ethanol extract (CEE), petroleum ether-and chloroform soluble-parts (PE and CE, resp.) inhibited growth of Gram-positive (Staphylococcus aureus, Baicllus licheniformis and Streptococcus agalactiae) and Gram-negative (Escherichia coli,Klebsiella pneumonia and Pseudomonas aeruginosa) with MICs ranging from 312.5 mg · L^-1 to 1 250 mg · L^-1. The MBCs of CEE against B. licheniformis, S. agalactiae and E. coli were 1 250 mg · L^-1, while those of PE and CE against all the test strains varied from 625 mg · L^-1 to 1 250 mg · L^-1. Conclusion: The CEE, PE and CE from the rhizomes of Z. striolatum possess antibacterial and bacteriocidal activities against S. aureus, B. licheniformis, S. agalactiae, E. coli,K. pneumonia and P. aeruginosa.