地榆升白片对环磷酰胺致小鼠骨髓抑制的拮抗作用

目的:观察地榆升白片对环磷酰胺所致小鼠骨髓抑制的保护作用.方法:Balb/C小鼠24只随机分为空白对照组、模型组、地榆升白片组.腹腔注射环磷酰胺100 mg· kg-1建立骨髓抑制模型,地榆升白片组按100 mg·kg-1灌胃,给药10d.观察地榆升白片对模型小鼠外周血象、骨髓有核细胞数、骨髓中DNA含量的影响,流式细胞术检测骨髓中各时相细胞周期百分比.结果:模型组小鼠外周血白细胞数、血小板数、骨髓有核细胞数以及骨髓中DNA含量显著降低(P＜0.05或P＜0.01)；骨髓细胞周期中G0/G1期细胞百分比显著增高,G2/M期细胞百分比显著降低(P＜0.05或P＜0.01).地榆升白片可以显著增加模型小鼠外周血白细胞数、骨髓有核细胞数及骨髓中DNA含量,恢复骨髓中各期细胞百分比(P＜0.05或P＜0.01).结论:地榆升白片对环磷酰胺致骨髓抑制有显著的改善和恢复作用.

Effect of Diyu Shengbai Tablets on Bone Marrow Depression Induced by Cyclophosphamide in Mice

Objective: To investigate the poctect function of Diyu Shengbai tabletts on bone marrow depression induced by cyclophosphamide in mice. Method: Twenty-four Balb/C mice, half male and femal, were categorized into three groups. Mice in model group and Diyu Shengbai group were administered cyclophosphamide (Cy) (100 mg · kg^-1 once a day for 3 days by intraperitoneal injection) to induce marrow suppression. Diyu Shengbai group was received Diyu Shengbai table treatment (100 mg · kg^-1body weight orally once a day for 10 days). A vehicle treated control group was also arrangeed. The changes of peripheral hemogram, bone marrow nucleated cells (BMCs), DNA content and bone marrow cell cycle were detected. Result: Cy-treated mice showed a significant decrease in the number of white blood cells, thrombocyte, bone marrow nucleated cell proliferation and the content of DNA in bone (compared with control group, there were significant differences,P<0.05 or P<0.01).The percentage of G₀/G₁ phase was increased and the percentage G₂/M phase decreased (compared with control group, there were significant differences,P<0.05 or P<0.01). Diyu Shengbai tablets significantly increased the number of white blood cells, thrombocyte, bone marrow nucleated cell proliferation and markedly increased the percentage of G₂/M phase(compared with model group (P<0.05 or P<0.01). Conclusion: Diyu Shengbai Tablets could improve the hemopoietic function for marrow suppression induced by Cy in mice.