结核病患者外周血T淋巴细胞CD28/CTLA-4表达及意义

目的分析结核病患者外周血共刺激分子CD28/CTLA-4(CD152)表达,评价患者的免疫状态。方法采集21例初诊结核病患者及15例健康对照者外周血,采用多参数流式细胞术检测分析共刺激分子CD28、CD152的表达,以及分析其亚群CD4+CD152+细胞、CD4+CD28+细胞、CD8+CD28+细胞、CD8+CD152+水平的变化。结果与健康者比较,结核病患者外周血CD28+细胞、CD152+细胞水平明显增高(P0.01)。对共刺激分子CD28、CD152进行亚群分析,结核病患者外周血CD4+CD152+细胞、CD4+CD28+细胞、CD8+CD152+细胞水平明显高于健康者(P0.05),CD8+CD28+细胞水平无统计学意义(F=2.647,P=0.113)。结论结核病患者外周血共刺激分子CD28/CD152(CT LA-4)表达增加,且CT LA-4在CD4+和CD8+T细胞上表达均增加,提示可能存在T细胞活化或抑制信号调节失衡。

Expression and significance of CD28/ CTLA-4 in peripheral blood T lymphocytes of patients with tuberculosis

Objective To investigate the expression of co-stimulatory molecules CD28 and cyto-toxic T-lymphocyteassociated antigen- 4（CTLA-4,CD152） in T lymphocytes from patients wit tuberculosis（TB）, and evaluate the immune state of TB patients. Methods Blood samples were collected from 21 TB patients and 15 healthy controls.The expression of costimulatory molecules CD28 and CD152 were determined, and the changes of subsets CD4＋CD152＋, CD4＋CD28＋, CD8＋CD152＋,CD8＋CD28＋were analyzed with flow cytometry and monoclonal antibodies technology.Results The percentages of CD28＋and CD152＋cells in TB patients were higher than in the healthy controls（P 0.01）. The percentages of CD4＋CD152＋, CD8＋CD152＋,CD4＋CD28＋cells in the TB patients were higher than in healthy controls（P 0.05）,without showing statistical significance in CD8＋CD28＋cells between TB patients and healthy controls（F=2.647,P =0.113）.Conclusion The expression of co-stimulatory molecules CD28/CD152（CTLA-4） was higher in TB patients.The abnormal expression of CD28 and CD152 indicates that coordination between activating and suppressing signals is destroyed.