住院腹泻患儿粪便艰难梭菌毒素检测与分析

目的对住院腹泻患儿的粪便标本进行艰难梭菌毒素筛查,以了解患儿艰难梭菌的感染情况,为院内感染的防控提供基础数据。方法采用VIDAS艰难梭菌AB毒素(CDAB)检测试剂对2013年6月~11月收集的146例住院腹泻患儿粪便标本进行毒素检测,并结合其他实验室检测结果及临床资料进行综合分析。结果 146例患儿中有10例检出CDAB,阳性率6.8%。10例CDAB阳性患儿均使用过美洛西林/舒巴坦、头孢他啶、头孢克洛等抗生素,占抗生素相关性腹泻患者(56例)的17.9%。结论住院患儿艰难梭菌相关性腹泻感染率较高,抗菌药物的使用是诱使艰难梭菌感染的重要因素,年幼体弱的儿童更容易感染艰难梭菌。

Detection and analysis of Clostridium difficile toxin in feces of hospitalized children with diarrhea

Objective To investigate status of Clostridium difficile infection in hospitalized children with diarrhea by screening Clostridium difficile in stool specimens and provide basic evidence for making measures of preventing and controlling nosocomial infection. Methods Faecal speeiments from 146 diarrhea inpatients in Guangdong Provincial Maternal and Child Healthcare Hospital were tested by the VIDAS Clostridium difficile toxins A ＆ B （CDAB）assay, the results obtained were epidemiologically analyzed . Results Among 146 diarrhea patients,10 were positive for CDAB with the positive rate of 6.8%. Patients with CDAB were treated with one or more antimicrobial agents, including mezlocillin/sulbactam, ceftazidime, cefaclor and other antibiotics, accounted for 17.9% （10/56） of patients with antibiotic-associated diarrhea.Conclusion C. difficile --associated diarrhea is relatively high in this hospital, antimicrobial use is an important factor resulting in C. diffcile infection, weak and young children are more susceptible to Clostridium difficile.