HPLC法同时测定野葛花中4种异黄酮成分的含量

目的建立野葛花中葛花苷、尼泊尔鸢尾异黄酮、鸢尾黄素-7-O-木糖葡萄糖苷、鹰嘴豆芽素A4种异黄酮成分含量测定方法。方法采用HPLC法。色谱柱为Kromasil C18柱(200 mm×4.6 mm,5μm),以体积分数为1%的乙酸水溶液-甲醇-乙腈为流动相,线性梯度洗脱,流速为0.8 mL.min-1,检测波长为254 nm,柱温35℃。结果葛花苷、尼泊尔鸢尾异黄酮、鸢尾黄素-7-O-木糖葡萄糖苷、鹰嘴豆芽素A质量浓度分别在42.8~856 mg.L-1、9.12~182.4 mg.L-1、13.2~264 mg.L-1、4.20~840 mg.L-1内呈良好线性关系,相关系数分别为0.999 9、0.999 9、0.999 1、0.999 3,平均回收率分别为100.7%(RSD=2.2%,n=9)、99.1%(RSD=2.4%,n=9)、98.6%(RSD=1.6%,n=9)和98.3%(RSD=1.5%,n=9)。结论该方法简便、准确、重现性好,可用于野葛花的质量控制。不同花期和不同药用部位的野葛花中4种指标成分的含量差异较大。

Simultaneous determination of four isoflavone compounds in flowers of Pueraria lobata by HPLC

Objective To develop a HPLC method for simultaneous determination of kakkalide, irisolidone, tectorigenin-7-O-xylosylglucoside and biochanin A in flowers of Pueraria lobata. Methods The HPLC analysis was carried out on a Kromasil C₁₈ (200 mm×4.6 mm, 5 μm) column , with a linearity gradient of 1% acetic acid in water（φ）- methanol - acetonitrile, at the flow rate of 0.8 mL·min^-1. The detective wavelength was set at 254 nm, and the column temperature was set at 35 ℃. Results The calibration curve was linear within the range of 42.8-856 mg·L^-1 for kakkalide, 9.12-182.4 mg·L^-1 for irisolidone, 13.2-264 mg·L^-1 for tectorigenin-7-O-xylosylglucoside and 4.2-84 mg·L^-1 for biochanin A, respectively. The average recoveries for four marker compounds were from 98.3% to 100.7%. Conclusions This method is simple, accurate and practical for quality control of flowers of Pueraria lobata. There are some differences in the contents of four marker compounds during the different flowering periods and between different medicinal parts.