孕三烯酮对大鼠血清雌孕激素及在位和异位子宫内膜雌孕激素受体的影响

目的:探讨孕三烯酮(gestrinone)治疗子宫内膜异位症的作用机理。方法:用自体移植术建立大鼠子宫内膜异位症动物模型,随机分组给药,3周后剖腹,取血测定雌二醇(E2)、孕酮(P)浓度,取出子宫及移植物,固定、切片,免疫组化SP法测定各组动物子宫及移植物的雌激素受体(ER)和孕激素受体(PR)的表达。结果:孕三烯酮高剂量使大鼠血清E2、P水平降低,而中低剂量对E2、P水平影响不大。免疫组化结果则显示移植物的ER、PR含量较子宫内膜低,孕三烯酮高、中剂量可下调在位和异位子宫内膜ER、PR表达,而低剂量孕三烯酮则不能明显改变ER、PR的表达量。结论:孕三烯酮可通过降低体内雌孕激素水平以及下调在位及异位子宫内膜ER、PR的表达,发挥对子宫内膜异位症的治疗作用。

Effects of Gestrinone on Levels of Serum Estradiol, Progesterone and Expressions of Estrogen Receptors, Progesterone Receptors in Eutopic and Ectopic Endometriums of Rats with Experimental Endometriosis

Objective: To explore the mechanism of action of gestrinone in the treatment of endometriosisby investigating the effects of gestrinone on the levels of serum E2, P and the expressions of estrogen receptors (ER)and progesterone receptors (PR) in eutopic and ectopic endometriums of rats with experimental endometriosis.Methods: Rats with experimental endometriosis induced by surgically autotransplanting were randomly divided intoseveral groups. After daily oral administrations of gestrinone for 3 weeks, the rats in different groups werelaparotomized again, blood samples were collected to measure levels of serum E2, P, the uteri and explants wereexcised to compare the expressions of ER and PR using immunohistochemical methods. Results: Decreases ofserum E2, P levels can be observed after treatments with high dose of gestrione, but similar findings were notobtained in low and middle dose groups. Lower receptor concentrations especially the concentration of ER werefound in ectopic endometrium than in eutopic endometrium. Immunohistochemical studies demonstrated that highand middle dose of gestrinone reduced the staining intensity of ER and PR in endometriotic and endometrial tissue.Conclusion: Gestrinone can decrease the levels of serum E2, P and reduce the expressions of ER, PR in botheutopic and ectopic endometriums, which maybe responsible for its treating effect on endometriosis.