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The evaluation of a user-friendly lateral flow assay for the serodiagnosis of human brucellosis in Kazakhstan |
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| Authors: | Sulushash Mizanbayeva Henk L Smits Katya Zhalilova Theresia H Abdoel Stanislaw Kozakov Kenes S Ospanov Philip H Elzer James T Douglas |
| Institution: | 1. Republican Sanitary Station and Epidemiological Station, Health Ministry of Kazakhstan Republic, 05008, 84 Auezov Street, Almaty, Kazakhstan;2. KIT Biomedical Research, Royal Tropical Institute / Koninklijk Institute voor de Tropen (KIT), Melbergdreef 39, 1105 AZ Amsterdam, The Netherlands;3. Veterinary Science and Veterinary Immunology, LSU Dep. Veterinary Science, 111 Dalrymple Building, Baton Rouge, LA 70803, USA;4. Department of Microbiology, University of Hawaii at Manoa, 2538 McCarthy Mall Snijder 207, Honolulu, HI 96822, USA |
| Abstract: | Serum samples from all patients with culture-confirmed brucellosis including those with chronic disease from Kazakhstan tested positive in the serum agglutination test for titers ≥1:25 and reacted in the Brucella immunoglobulin M/immunoglobulin G lateral flow assay (LFA) confirming the high sensitivity of these assays. The strong reactivity in the LFA observed for the majority (92.1%) of the samples from the patients with culture-confirmed brucellosis together with the user-friendliness of the assay procedure makes the LFA ideal for the confirmation of brucellosis in endemic areas in Kazakhstan. The Rose Bengal test lacked sensitivity in particular for patients with chronic brucellosis therefore limiting its value as a quick screening assay. The study emphasizes the importance of the LFA as a useful, rapid, and easy-to-perform tool in the diagnostic testing of brucellosis. |
| Keywords: | Brucellosis Serodiagnosis Culture Acute Chronic Kazakhstan |
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