曲克芦丁对大鼠肝脏缺血再灌注损伤的保护作用

目的探讨曲克芦丁对大鼠肝脏缺血再灌注损伤的影响及其机理。方法将W istar大鼠40只随机分为假手术对照组、缺血再灌注组(I/R组)和I/R加曲克芦丁预处理组。I/R加曲克芦丁预处理组动物术前腹腔内注射曲克芦丁20 mg/kg,1次/d,连续3 d。然后制作大鼠肝缺血再灌注损伤模型,检测大鼠血清中谷丙转氨酶(ALT)和谷草转氨酶(AST)的含量及肝组织中脂质过氧化反应产物丙二醛(MDA)、超氧化物歧化酶(SOD)的含量,并通过光镜观察肝组织结构的形态学改变。结果用曲克芦丁处理组大鼠血清中ALT和AST及肝组织中MDA含量明显低于缺血再灌注组(P<0.01),而肝组织中SOD含量则高于缺血再灌注组(P<0.01),与假手术组差异较小(P<0.05),肝细胞形态学异常改变较I/R组也明显减轻。结论曲克芦丁对肝脏I/R损伤具有保护作用,其机理与抑制氧自由基的生成,增强氧自由基的清除,减轻肝细胞脂质过氧化作用有关。

Preventive Effect of Troxerutin Injection on the Damage of Ischemia-Reperfusion in Rat Liver

Objective To study the preventive effect on damage of ischemia-reperfusion in rat liver by applying Troxerutin Injection.Methods30 rats were put randomly into sham operated control(SOC)group,ischemia reperfusion(I/ R) group and ischemia reperfusion Troxerutin(I/ R Troxerutin)group.The animal model of hepatic ischemia-reperfusion was made by temporarily blocking the hepatic portal blood vessels and releasing 30 minutes later.The malondialdehyde(MDA),superoxide dismutase(SOD) in liver tissue and ALT,AST levels in the blood were determined after the liver had been reperfused for 120 minutes,the hepatic tissue was examined under light microscope.ResultsMDA,ALT,AST decreased and SOD increased significantly in I/ R Troxerutin group when compared those in the I/ R group(P< 0.01).The histologic changes of the liver tissue under light microscope in the I/ R Troxerutin group was more easer than in the I/ R group.ConclusionTroxerutin has the ability to scavenge oxygen free radical(OFR) and to protect from the damage of ischemia-reperfusion in rat liver.